Protein Purification Techniques 电子书 读后感 评分☆☆☆ 评分☆☆☆ 评分☆☆☆ 评分☆☆☆ 评分☆☆☆ 类似图书 点击查看全场最低价 出版者:Oxford Univ Pr作者:Roe, Simon 编出品人:页数:278译者:出版时间:2001-1价格:$ 141.25装帧:Papisbn...
Efficient purification is crucial to providing large quantities of recombinant therapeutic proteins, such as monoclonal antibodies and cytokines. However, affinity techniques for manufacturing protein therapeutics that use biomolecule-conjugated agarose beads that harness specific biomolecular interactions suffer fro...
Ni+-affinity purification of untagged cAMP receptor protein. The cAMP receptor protein (CRP, also known as CAP) functions as a transcriptional activator at more than 100 different E. coli operons (3). CRP is arguably... Wickstrum, Jason R.,Egan, Susan M. - 《Biotechniques》 被引量: 25...
Purification Techniques ...20 Sample Conditioning ...26 Chapter 4 Capture ...29 Chapter 5 Intermediate Purification ...
* Something else to know about hemoglobin Hemoglobin binds CO with higher affinity; Sickle-cell anemia is caused by hemoglobin mutation E?6V. * ATP synthase is a highly complex molecular machine * * * Basic principle of protein purification * protein purification techniques Based on physical and ...
purification step is necessary to reach the desired purity. The key to successful and efficient protein purification is to select the most appropriate techniques, optimise their performance to suit the requirements and combine them in a logical ...
Overview of Affinity Tags for Protein Purification Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the b... ME Kimple,AL Brill,RL Pasker - 《Current Protocols in Protein Science》 被引量: 57发表:...
Affinity purification can be used to purify a specific kind of molecule (positive selection) or to remove a specific kind of contaminant (negative selection). Both methods often involve buffer exchange. Affinity purification involves the...
Magnetic beads and KingFisher Purification Systems enable the automation of immunoprecipitation (IP) and other affinity purification protocols for proteomics analysis by mass spectrometry. Learn more Featured data Enrichment using streptavidin magnetic beads...
Ecolab offers comprehensive solutions in affinity chromatography for mAbs and recombinant proteins. Ideal for advanced purification techniques.