Biochemical, biophysical, and structural studies of membrane proteins rely on the availability of highly pure and monodisperse membrane protein samples. One of the most powerful methods for isolation...doi:10.1007/978-1-0716-0373-4_9Elisabeth Graeber...
Three proteins with clinic value, gamma-globulin, plasminogen and thrombin, were purified from human plasma by one step with affinity membrane chromatography. The purities of gamma-globulin purified were higher than 83%, and purification folds were higher than 5. The purity of gamma-globulin ...
Expression of a prokaryotic P-type ATPase in E. coli Plasma Membranes and Purification by Ni2+-affinity chromatographyCa(2+)-Transporting ATPase... M Geisler - 《Biological Procedures Online》 被引量: 1发表: 1998年 Purification and Identification of a Plasma Membrane Associated Electron Transport ...
Monoclonal antibody affinity purification of a 78 kDa membrane protein of Leishmania donovani of Indian origin and its role in host-parasite interaction. Swadesh Duttagupta.Monoclonal antibody affinity purification of a 78 kDa membrane protein ofLeishmania donovani of Indian origin and its role in ...
Efficient purification is crucial to providing large quantities of recombinant therapeutic proteins, such as monoclonal antibodies and cytokines. However, affinity techniques for manufacturing protein therapeutics that use biomolecule-conjugated agarose beads that harness specific biomolecular interactions suffer fro...
Especially in combination with immobilized Strep-Tactin, a mutant streptavidin specifically optimized toward the Strep-tagII, this system enables the facile one-step affinity purification of various biomolecules, including oligomeric and even membrane proteins. The Strep-tagII/Strep-Tactin interaction shows...
E. (2013) Development of a novel affinity membrane purification system for deoxyribonuclease. Applied biochemistry and biotechnology.Landry, K. S., & Levin, R. E. (2014). Development of a novel affinity membrane purification system for deoxyribonuclease. Applied Biochemistry and Biotechnology, 172, ...
Proteomic analysis of integral plasma membrane proteins. Here we report a biotin-directed affinity purification (BDAP) method for the preparation of integral plasma membrane proteins, which involves (1) ... Y Zhao,W Zhang,Y Kho,.....
purification metabolismChromatography, Affinity methodsEscherichia coli genetics metabolismThe maltose transport system of Escherichia coli, a member of the ABC ... AL Davidson,S Sharma - 《Journal of Bacteriology》 被引量: 182发表: 1997年 Actions of cadmium on basolateral plasma membrane proteins invol...
(1988) Membrane-Based Affinity Technology for Commercial Scale Purifications, Bio/Technol. 6 , 779–782.Brandt S, Goffe RA, Kessler SB, O-Connor JL & Zale SE (1988) Membrane-based affinity technology for commercial scale purifications. Bio/Technology 6(7): 779–782....