In the present study, 70K protein was partially purified using affinity Sepharose on which membrane proteins of NC4 cells were immobilized. Through this process, involvement of Ca 2+ in the interaction of 70K p
Three proteins with clinic value, gamma-globulin, plasminogen and thrombin, were purified from human plasma by one step with affinity membrane chromatography. The purities of gamma-globulin purified were higher than 83%, and purification folds were higher than 5. The purity of gamma-globulin ...
The separated proteins can then be probed with antibodies; this variation of the technique is known as immunoblotting (or western blotting). The membrane can also be probed with specific ligands, such as DNA, protein, small molecules (for example, heparin or GTP) or even whole cells. ...
Affinity purification of sucrose binding proteins from the plant plasma membrane. Biochim. Biophys. Acta 1219:11 389 – 397 .Li Z, Noubhani A, Bourbouloux A, Delrot S. 1994. Affinity purification of sucrose binding proteins from the plant plasma membrane. Biochimica et Biophysica Acta 1219, ...
Efficient purification is crucial to providing large quantities of recombinant therapeutic proteins, such as monoclonal antibodies and cytokines. However, affinity techniques for manufacturing protein therapeutics that use biomolecule-conjugated agarose beads that harness specific biomolecular interactions suffer fro...
E. (2013) Development of a novel affinity membrane purification system for deoxyribonuclease. Applied biochemistry and biotechnology.Landry, K. S., & Levin, R. E. (2014). Development of a novel affinity membrane purification system for deoxyribonuclease. Applied Biochemistry and Biotechnology, 172, ...
Expression and Purification of Fusion Proteins— BL21(DE3) cells, harboring the different expression constructs, were inoculated into culture medium as described before (13) and induced at A600 nm≈ 1.0 with isopropyl-β-d-thiogalactopyranoside (Sigma Aldrich, St. Louis, MO) at a final concentrati...
FurtherpurificationofalliinasewasconductedonpreparedaffinitychromatographycolumnanditspuritywasexaminedbySDS-PAGE. 用制得的亲和胶分离纯化,并利用SDS-PAGE对酶纯度进行检验。 124.205.222.100 5. Serumpretreatedwithcouplingaffinitychromatographyisbetterthanmembraneabsorption. ...
The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease...
(1988) Membrane-Based Affinity Technology for Commercial Scale Purifications, Bio/Technol. 6 , 779–782.Brandt S, Goffe RA, Kessler SB, O-Connor JL & Zale SE (1988) Membrane-based affinity technology for commercial scale purifications. Bio/Technology 6(7): 779–782....