Biochemical, biophysical, and structural studies of membrane proteins rely on the availability of highly pure and monodisperse membrane protein samples. One of the most powerful methods for isolation...doi:10.1007/978-1-0716-0373-4_9Elisabeth Graeber...
Three proteins with clinic value, gamma-globulin, plasminogen and thrombin, were purified from human plasma by one step with affinity membrane chromatography. The purities of gamma-globulin purified were higher than 83%, and purification folds were higher than 5. The purity of gamma-globulin ...
Proteomics analysis of plasma membranes is a potentially powerful strategy for the discovery of proteins involved in membrane remodeling under diverse cell... Y Zhao,W Zhang,MA White,... - 《Analytical Chemistry》 被引量: 83发表: 2003年 Characterization and partial purification of multiple electron...
Affinity Purification of Specific Antibodies from Plant Virus Capsid Protein Immobilised on Nitrocellulose The application of a small-scale method for the preparation of antibodies specific for coat proteins of simple plant viruses is described. The method entai... EP Rybicki - 《Journal of Phytopatholo...
Purification of the major outer membrane protein of Azospirillum brasilense, its affinity to plant roots, and its involvement in cell aggregation The major outer membrane protein (MOMP) of the nitrogen-fixing rhizobacterium Azospirillum brasilense strain Cd was purified and isolated by gel filtration...
High-capacity purification of His-tagged proteins by affinity membranes containing functionalized polymer brushes. Porous membrane absorbers are attractive for increasing the rate of protein purification, but their binding capacity is low relative to porous beads. Modif... P Jain,L Sun,J Dai,... -...
Affinity purification of sucrose binding proteins from the plant plasma membrane. Biochim. Biophys. Acta 1219:11 389 – 397 .Li Z, Noubhani A, Bourbouloux A, Delrot S. 1994. Affinity purification of sucrose binding proteins from the plant plasma membrane. Biochimica et Biophysica Acta 1219, ...
Efficient purification is crucial to providing large quantities of recombinant therapeutic proteins, such as monoclonal antibodies and cytokines. However, affinity techniques for manufacturing protein therapeutics that use biomolecule-conjugated agarose beads that harness specific biomolecular interactions suffer fro...
E. (2013) Development of a novel affinity membrane purification system for deoxyribonuclease. Applied biochemistry and biotechnology.Landry, K. S., & Levin, R. E. (2014). Development of a novel affinity membrane purification system for deoxyribonuclease. Applied Biochemistry and Biotechnology, 172, ...
Purification and biochemical characterization of proteins which bind to the H-boxcis-elementimplicated in transcriptional activation of plant defense genes SummaryThe H-box (CCTACC(N)7CT(N)4A), which occurs three times within the -1...