我们描述包含细菌免疫多肽的融合蛋白及其在蛋白复合物的亲和纯化中的用途. We describe a fusion protein bacterial immunogenic polypeptide and their use in affinity purification of the protein complex.科林·克莱安杜斯西奥内·乔治乌Fahrner R, Follman D, Lebreton B, Van Reis R. United States Patent 2008...
申请(专利权)人: THE UNIVERSITY OF YORK;KLEANTHOUS, COLIN;GEORGIOU, THEONIE 发明人:C Kleanthous,T Georgiou 被引量: 4 摘要: We describe fusion proteins comprising a bacterial immunity polypeptide and their use in affinity purification of protein complexes....
Development of operating conditions for protein purification using expanded bed techniques: The effect of the degree of bed expansion on adsorption performance 1996, Biotechnology and Bioengineering Membrane Chromatography—An Integrative Concept in the Downstream Processing of Proteins 1995, Biotechnology Progre...
The use of expanded beds of affinity adsorbents for the purification of proteins from feedstocks containing whole or broken cells is described. It is demonstrated that such feedstocks can be applied to the bed without prior removal of particulate material by centrifugation or filtration thus showing ...
protein of interest from other proteins and components in a crude cell lysate or other sample. The most powerful of these methods is affinity chromatography, also called affinity purification, whereby the protein of interest is purif...
limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low expression levels are employed. Here, we discuss the advantages and restrictions of tagging recombinant proteins with histidines and purifying them by Ni2+-NTA ...
Affinity purification is a technology that enriches or purifies a protein from a complex mixture of biomolecules such as a cellular lysate, cell culture supernatant or serum etc. Nanobodies are especially well suited for affinity purification, as they co
protein of interest from other proteins and components in a crude cell lysate or other sample. The most powerful of these methods is affinity chromatography, also called affinity purification, whereby the protein of interest is purified ...
Efficient purification is crucial to providing large quantities of recombinant therapeutic proteins, such as monoclonal antibodies and cytokines. However, affinity techniques for manufacturing protein therapeutics that use biomolecule-conjugated agarose beads that harness specific biomolecular interactions suffer fro...
protein material. However, it allows the purification of only one protein at a time. To decipher the protein–protein interactions of a panel of E6 oncoproteins, parallel purifications would be more appropriate. In addition, a faster purification protocol would reduce the risk of protein ...