Ecolab offers comprehensive solutions in affinity chromatography for mAbs and recombinant proteins. Ideal for advanced purification techniques.
Affinity chromatography involves targeted purification of biological macromolecules from a crude mixture on the basis of highly specific interaction between the macromolecule and a tag protein or peptide. The interaction is typically reversible and purification is implemented by keeping one of the molecules...
Most commonly, affinity purification is done either as batch purification or via a column packed with the affinity resin (affinity chromatography). Nanobodies are especially well suited for affinity purification, as they combine both high selectivity with a high degree of robustness. Since Nanobodies...
Custom Protein Development Services have frequently applied affinity chromatography technology to produce large-scale amounts of recombinant membrane protein based on custom protein expression and purification platform.
Various methods are used to enrich or purify a protein of interest from other proteins and components in a crude cell lysate or other sample. The most powerful of these methods is affinity chromatography, also called affinity pur...
To purify a protein with the help of their affinity to another molecule, the interacting partner (ligand), e.g., a protein, a small molecule, or a metal, is immobilized on the stationary phase of the chromatography matrix. The stationary phase mostly consists of agarose or synthetic polymers...
Affinity chromatography relies on the specific and reversible binding of a protein to a matrix-bound ligand. The ligand can bind directly to either the protein of interest or a tag that is covalently attached to the protein. Affinity chromatography is often the most robust purification procedure an...
Various methods are used to enrich or purify a protein of interest from other proteins and components in a crude cell lysate or other sample. The most powerful of these methods is affinity chromatography, also called affinity purifica...
Thus, affinity chromatography allows purification of most proteins to near homogeneity by utilizing the specific structure of the protein (for reviews see Scouten 1981; Wilchek et al. 1984).doi:10.1007/978-3-642-59219-5_2H. WombacherL. Jacob...
Therecombinantproteinwasexpressedmainlyin theformofinclusionbodiesandcould behighlypurifiedbyNi2+affinitychromatography. 重组蛋白表达主要以包涵体形式存在,经亲和层析纯化后获得了高纯度的重组蛋白; www.fabiao.net 4. Furtherpurificationofalliinasewasconductedonpreparedaffinitychromatographycolumnanditspuritywasexaminedby...