Principle of affinity-based protein purification Comparison of direct and indirect affinity purification strategies Due to the highly specific selection and the resulting purity of target proteins, affinity-based systems have become the flagship method of purification. However, some of the widely used aff...
Affinity chromatography allows for the separation of biomolecules such as proteins, based on a change in the chemical solvent composition and the resulting impacts on ligand binding. Here, authors introduce a physical principle by exploiting the light-dependent interaction between the Azo-tag and an ...
In principle, metaproteome can be employed to simultaneously determine the protein composition of microbiome and host. However, in practice, this can be challenging due to the high microbial species diversity and high sequence homology at the protein level in particular between closely related species...
Yoichiro Ito.Centrifugal Precipitation Chromatography: Principle, Apparatus, and Optimization of Key Parameters for Protein Fractionation by Ammonium Sulfate Precipitation. Analytical Biochemistry . 2000Ito Y. Anal Biochem. 2000; 277 :143.Ito Y (2000) Centrifugal precipitation chromatography: principle, appa...
(https://www.agilent.com/store/en_US/Prod-200131/200131)). Proteins were purified by NTA affinity chromatography, taking advantage of the presence of a His-tag at the C-terminal. Protein solutions were prepared by exhaustive dialysis against 50 mM HEPES buffer and protein concentrations were...
Design of Ligands for Affinity Purification of G-CSF Based on Peptide Ligands Derived from a Peptide Library Combinatorial peptide libraries have become powerful tools to screen functional ligands by the principle of affinity selection. We screened in a phage pept... R Fang,ZHANG Chng-dong,LP Wan...
Molecular docking as a powerful method for examining phenolic-protein interactions Molecular docking is an in-silico computational tool that can be used to predict the preferred orientation, affinity, and interaction of a phenolic compound in the binding site of a protein. This method allows for com...
Despite their lack of a defined 3D structure, intrinsically disordered regions (IDRs) of proteins play important biological roles. Many IDRs contain short linear motifs (SLiMs) that mediate protein-protein interactions (PPIs), which can be regulated by p
The results demonstrate that the principle of natural recognition found in the lock-and-key motif, in combination with de novo combinatorial design, may lead to synthetic affinity ligands, useful in downstream processing and proteomic research. 展开 ...
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248–254. [Google Scholar] [CrossRef] Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4...