Principle of Affinity Chromatography The stationary phase consists of a support medium, on which the substrate (ligand) is bound covalently, in such a way that the reactive groups that are essential for binding of the target molecule are exposed. As the crude mixture of the substances is passed...
The principle of affinity chromatography was used for preparation of thromboresistant bilayer coatings. The outer biospecific layer containing ε-aminocaproic acid residues (from 2.2 up to 5.5 nmol/cm 2) was synthesized using a copolymer of maleic anhydride with N-vinylpyrrolidone and l-lysine ...
A new approach to the management of familial hypercholesterolaemia: Removal of plasma-cholesterol based on the principle of affinity chromatography. (LOS), A New Approach to the Management of Familial Hypercholesterolaemia: Removal of Plasma-Cholesterol Based on the Principle of Affinity Chromatography...
Ion exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their affinity to ion exchangers. The principle of separation is thus by reversible exchange of ions between the target ions present in the sample solution to the ions ...
CompoundsBiomoleculesLiquid ChromatographyStationary-phase BeadsResinAffinityCation-exchange ChromatographyAnion-exchange ChromatographyUnbound CompoundsProtein MixtureLaboratoryStrong Cation-exchange ResinsWeak Cation-exchange ResinsStrong Anion-exchange ResinsWeak Anion-exchange ResinsSample MixtureAnalyte Of InterestBuffers...
Taber, D. F.; Hoerrner, R. S., Column chromatography: Isolation of caffeine.Journal of Chemical Education, 68(1), 73 (1991). Transcript 1.硅胶浆 硅凝胶倒入锥形瓶。包装材料的重量应该是大约 50 x 被分离的样品。如果被分离的化合物有很相似的Rf值,它可能需要使用更大数额的每个样品,在此示例中...
The exchange affinity of various ions is a function of the net surface charge. Therefore, the efficiency of the ion exchange process for As(V) removal depends strongly on the solution pH and the concentration of other anions, most notably sulfates and nitrates. High levels of total dissolved ...
(https://www.agilent.com/store/en_US/Prod-200131/200131)). Proteins were purified by NTA affinity chromatography, taking advantage of the presence of a His-tag at the C-terminal. Protein solutions were prepared by exhaustive dialysis against 50 mM HEPES buffer and protein concentrations were...
The developed lectin microarray system allows even researchers with poor experience in glycan profiling to perform extensive high-throughput analysis targeting various forms of glycans and even cells. 展开 关键词: FRONTAL AFFINITY-CHROMATOGRAPHY CARBOHYDRATE-BINDING-SPECIFICITY PLURIPOTENT STEM-CELLS ...
The equilibrium for gas chromatography is partitioning, and the components of the sample will partition (i.e. distribute) between the two phases: the stationary phase and the mobile phase. Compounds that have a greater affinity for the stationary phase spend more time in the column and thus elu...