This protein is widely used as a ligand for affinity chromatography to purify therapeutic antibodies on an industrial scale. This type of affinity chromatography constitutes a generic step in processing antibodies. Questions of scale-up, design of chromatographic conditions, clearance of adventitious ...
Protein A chromatography is one of the most widely used purification steps in the manufacturing of the various classes of recombinant and non-recombinant antibodies. Due to the higher cost, lower binding capacity, and limited life cycle of Protein A ligand, this affinity-based purification step is...
We also investigated by pre-steady-state kinetic analysis, whether conformational changes of the protein are essential for effective binding of the bulky CTZ substrate (Fig.3b). The combination of analytical data processing and global fitting by numerical integration revealed a two-step induced fit s...
Protein purification with light via a genetically encoded azobenzene side chain Affinity chromatography allows for the separation of biomolecules such as proteins, based on a change in the chemical solvent composition and the resulting impacts on ligand binding. Here, authors introduce a physical principl...
The fusion protein, designated FN RGD /OC, was expressed in Escherichia coli and purified with affinity chromatography using a His-tag. The resultant FN RGD /OC fusion protein preferentially bound to HA, promoted cell adhesive activity, and stimulated differentiation of MC3T3-E1 cell. 展开 ...
By optimizing the protein structure of the WT, the maximum binding affinity (binding energy = −7.95 kcal/mol) was taken as a reference for subsequent evaluation and selection of variants. The mutants with lower binding energy scores than the WT and the one with the best binding ene...
To assess the effect of the knockouts on downstream purification, we performed protein A affinity chromatography, followed by cation exchange chromatography (CIEX) and anion exchange chromatography (AIEX) (Supplementary Fig.5). Samples were collected after every chromatographic step and were analyzed with...
Immobilized metal affinity chromatography (IMAC) allows for robust protein purification via affinity tags such as the hexahistidine (His(6)) sequence. Through the combination of a diverse AAV2 library and rational peptide insertions, we have located an optimal His(6) tag insertion site within the...
Systems in Membrane Reactors.- Scale-Up of Protein Purification by Liquid-Liquid Extraction.- Rapid and Large-Scale Purification of Angiotensin-I Forming Enzymes and Milk Clotting Enzymes by Affinity Chromatography.- Separation and Purification of Enzymes Via Continuous Parametric Pumping.- Session II. ...
Briefly, human p97 constructs were expressed with a His6 tag and purified by affinity chromatography and size-exclusion chromatography (SEC, using a Superose 6 10/300 column). Human p37 was expressed in Escherichia coli with an N-terminal glutathione-S-transferase (GST)-tag. The protein was ...