Lennarz WJ, Lane MD. 2013. Affinity tags for protein purification. In Ency- clopedia of biological chemistry, Falke JJ, Corbin JA (eds). University of Colorado Press: Boulder; 61-65.J.J. Falke, J.A. Corbin, Affinity tags for protein purification, in: William.J. Lennarz, M. Daniel ...
Limited effect on protein functionality unlike larger tags (GST, HIS6) High selectivity and binding affinity for recombinant protein purification Enabling high target yield and purity from complex mixtures (compared to HIS6-tag) CaptureSelect C-tagXL resin High binding...
Overview of Affinity Tags for Protein Purification Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the b... ME Kimple,AL Brill,RL Pasker - 《Current Protocols in Protein Science》 被引量: 57发表:...
Efficient purification is crucial to providing large quantities of recombinant therapeutic proteins, such as monoclonal antibodies and cytokines. However, affinity techniques for manufacturing protein therapeutics that use biomolecule-conjugated agarose beads that harness specific biomolecular interactions suffer fro...
These applications include affinity tags for protein purification, and non-chemical methods for immobilisation and presentation of recombinant protein domains on ... RE Nordon,SJ Craig,FC Foong - 《Biotechnology Letters》 被引量: 62发表: 2009年 Escherichia coli expression and purification of LL37 fus...
Thus, ICATs can be labelled with either a heavy or light isotope and contain a biotin tag for affinity purification. Initially, the mass difference between the tags was generated with deuterium [67], but this method has since been improved and the ‘heavy’ tag now contains nine 13C ...
For example, if a protein of interest interacts with other subunits through its C-terminal re- gion, fusion of affinity tags to the C terminus might inhibit these interactions. If a system that can express a protein of interest with affinity tags at multiple positions can be developed, ...
英文: Choosing of Affinity Tags for Recombinant Proteins and Application Progress of Tandem Affinity Purification中文: 重组蛋白质亲和标签的选择与串联亲和纯化的应用进展 英文: They share a special affinity.中文: 他们有共同的特殊爱好.英文: An Engineered Affinity Protein-affibody中文: 新型蛋白质配体-亲和...
The present study reviewed and discussed the promising affinity tags for one-step purification and immobilization of recombinant proteins. The approach use
Concentration Kit - GST Purification of GST-tagged recombinant proteins.Catalog Nos. ACR5000GS, ACK5003GS, ACK5010GS, ACK5030GS,ACK5050GS, ACK5100GS FOR RESEARCH USE ONLY Not for use in diagnostic procedures.USA & Canada Phone: +1(800) 437-7500 Fax: +1 (951) 676-9209 Australia +61 3...