Membrane protein (MP) functional and structural characterization requires large quantities of high-purity protein for downstream studies. Barriers to MP characterization include ample overexpression, solubilization, and purification of target proteins while maintaining native activity and structure. These ...
Cloning, expression and purification of orthologous membrane proteins: a general protocol for preparation of the histidine sensor kinase ETR1 from different species. Mol Membr Biol. 2012;29(2):26–35.Classen, E. & Groth, G. Cloning, expression and purification of orthologous membrane proteins: a...
Three proteins with clinic value, gamma-globulin, plasminogen and thrombin, were purified from human plasma by one step with affinity membrane chromatography. The purities of gamma-globulin purified were higher than 83%, and purification folds were higher than 5. The purity of gamma-globulin ...
Vuckovic, D.;et al.Membrane proteomics by high performance liquid chromatography-tandem mass spectrometry: Analytical approaches and challenges.Proteomics.2013. Thomas Arnold, Dirk Linke. Phase separation in the isolation and purification of membrane proteins.Biotechniques.2018....
Recently, some groups have reported on cell-free synthesis of functional membrane proteins (MPs) in the presence of exogenous liposomes (liposomes). Previously, we reported synthesis of a functional AtPPT1 plant phosphate transporter that was associated with liposomes during translation. However, it ...
Concentration Polarization with Membrane Ultrafiltration This report reviews the nomenclature of the milk proteins of cow's milk in light of more recent advances in our knowledge. With the establishment of the pr... M.,C.,Porter - 《Industrial & Engineering Chemistry Research》 被引量: 674发表:...
Charcosset C.Purification of proteins by membrane chromatography. Journal of Chemical Technology and Biotechnology . 1998CHARCOSSET C.Purification of proteins by membrane chro-matography. Journal of Chemical Technology and Biotech-nology . 1998D. Charcosset, Purification of proteins by membrane chromat-...
This manuscript reports a high-throughput platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs by using a library of membrane-active polymers while maintaining their local membrane context. Caroline Brown
Extraction of Membrane Proteins The landmark paper on biomembrane structure in 1972 by Singer and Nicolson ( 1 ) is still widely accepted as an excellent working hypothesis ( 2 ), but a m... P Cutler,K Ohlendieck - Humana Press 被引量: 33发表: 2004年 Biomimetic functionalization of porous...
Purification of the major outer membrane protein of Azospirillum brasilense, its affinity to plant roots, and its involvement in cell aggregation. The major outer membrane protein (MOMP) of the nitrogen-fixing rhizobacterium Azospirillum brasilense strain Cd was purified and isolated by gel filtration...