其中,该研究使用了DNA亲和纯化测序技术(DAP-seq,DNA Affinity Purification Sequencing),鉴定了水稻生物钟核心组分OsCCA1(Oryza sativa CIRCADIAN CLOCK ASSOCIATED 1)调控的下游靶基因。 2020年4月,北京工商大学与蓝景科信合作,Biochemical and Biophysical Research Communications(IF=3.322)发表了题为“Phytochrome-interactin...
该研究借助DNA亲和纯化测序(DNA Affinity Purification Sequencing,DAP-seq)技术,深入揭示了胡杨耐盐的分子机制。
DNA affinity purification sequencing (DAP-seq) couples in vitro expression of transcription factor (TF) and binding to a genomic DNA library with next-generation sequencing, providing a robust method to identify genome-wide transcription factor binding sites (TFBS) on endogenous genomic DNA. Here we...
然而MpRR-MYBs作为转录因子是如何实现对这些结构基因的直接影响的还不甚清楚。为此,作者们进行了DAP-seq(DNA affinity purification and sequencing)鉴定全基因组中MpRR-MYB5以及MpRR-MYB2在基因组中的结合位点以及它们所识别的DNA序列基序。作者们共鉴定出6804个MpRR-MYB2以及2839个MpRR-MYB5的结合位点。通过...
DAP-SEQ(DNA亲和纯化测序)体外蛋白表达载体构建PROTOCOL CAS号: 英文名: DAP-SEQ (DNA affinity purification sequencing) in vitro protein expression vector construction PROTOCOL 英文别名: DAP-SEQ (DNA affinity purification sequencing) in vitro protein expression vector construction PROTOCOL ...
为了探究LFY-UFO基因组结合位点,作者通过ampDAP-seq(amplified DNA affinity purification sequencing),发现600多个UFO-LFY特异的结合位点,表明UFO和LFY形成复合体,识别新的DNA顺式作用元件,调控基因表达(图1)。 图1 LFY和UFO协同激活AP3表达 此外,作者发现,LFYK249R突变不影响LFY原本活性,但抑制UFO-LFY与下游靶...
背景: DAP-seq: DNA affinity purification sequencing(DNA亲和纯化测序) AUXIN RESPONSE FACTORS (ARFs):plant-specifictranscription factors (TFs) that couple perception of the hormone auxin to geneexpression programs. highly conserved nuclear auxinsignal transduction pathway is composed of the TIR1/AFB-Aux...
进一步研究发现，LFY-UFO激活AP3转录的结合位点不同于经典的LFY结合位点。为了探究LFY-UFO基因组结合位点，作者通过ampDAP-seq（amplified DNA affinity purification sequencing），发现600多个UFO-LFY特异的结合位点，表明UFO和LFY形成复合体，识别新的DNA顺式作用元件，调控基因表达（图1）。图1 LFY和UFO协同激活AP3...
进一步研究发现,LFY-UFO激活AP3转录的结合位点不同于经典的LFY结合位点。为了探究LFY-UFO基因组结合位点,作者通过ampDAP-seq (amplified DNA affinity purification sequencing) ,发现600多个UFO-LFY特异的结合位点,表明UFO和LFY形成复合体,识别新的DNA顺式作用元件,调控基因表达 (图1) 。
(PCLs), one family of PRC2-associated factors23, to specific DNA sequence, which may affect transcription states, higher-order chromatin interactions and chromatin states24,25. A high-throughput TF-binding site discovery method, namely DNA affinity purification sequencing (DAP-seq), allows to ...