DNA Purification by Triple-Helix Affinity Precipitation. Biotechnol. Bioeng. 2003, 81, 535-545.Costioli, M., Fisch, I., Garret-Flaudy, F., Hilbrig, F. and Freitag R., 2003, DNA purification by triple helix affinity precipitation: The THAP process. Biotechnol Bioeng, 81(5): 535- 545...
Purification Affinity purification About the Immunogen Immunogen Recombinant protein Gene ID 5591(Human); 19090(Mouse); 360748 (Rat) SwissProt P78527(Human); P97313(Mouse) Synonyms DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; XRCC7; p350 Category Cell Cycle Background The phosphati...
A high-throughput assay that introduces mismatched base pairs into the DNA sequence shows that mismatches can increase transcription factor binding affinity by prepaying some of the energetic cost of distorting the DNA. Ariel Afek ,Honglue Shi ...
These assay designs can be applied to identify different types of functional relationships (Fig. 1c). By testing both possible AD orientations for each TF-pair (TF1-AD + TF2, TF1 + TF2-AD), the 1-AD design can be used to differentiate between two classes of cooperativity—...
Purification Affinity Chromatography Applications WB, ICC, IHC-P, IHC-F, ELISA Concentration 500ug/ml UOM 200ug Conjugate No Conjugate ANTIBODY SPECIFITY DNA损伤诱导转录因子3(DDIT3)单克隆抗体 The antibody is a mouse monoclonal antibody raised against DDIT3. It has been selected for its ability to...
3.2.1Bacterial one-hybrid assay The B1H method, developed by Meng et al.[38,84]and only requiring standard laboratory equipment, is in principle applicable to any TF that can be cloned and expressed inE. coli, with the advantage (similar to the Y1H) that the TF(s) of interest does not...
The COBAS AmpliPrep/COBAS TaqMan HCV is the first FDA-approved real-time RT-PCR assay for the quantification of HCV RNA. This fully automated assay is performed on two different systems, the COBAS AmpliPrep system, which performs extraction, purification, and reaction setup, and the COBAS TaqMa...
This assay also permits quantitative determination of the affinity, abundance, association rate constants, dissociation rate constants, and binding specificity of DNA-binding proteins. A supershift assay is a variation of the mobility shift DNA-binding assay that uses antibodies to identify proteins ...
Affinity Beads 亲和珠 100 l 4℃ DPS(DNA Purification Solution) DNA纯化溶液 600 l RT DNA Binding Beads DNA结合珠 60 4℃ Elution Buffer 洗脱缓冲液 1 ml RT *在开盖使用之前,将溶液离心至管底。 自备材料: 设备: 漩涡混合器 显微镜和细胞计数器 ...
To understand the autoimmune basis, the DNA abyzme activity mediated by serum polyclonal IgG Abs were examined in psychoses patients, quantitatively, by an in-house optimized DNase assay. A similar activity exhibited by IgG Abs from neuropsychiatric-systemic lupus erythematosus (NP-SLE) patients was...