flow cytometryDendritic cells (DCs) play a crucial role in the complex interplay between tumor cells and the immune system. During the elimination phase of cancer immunoediting, immunostimulatory DCs are critical for the control of tumor growth. During the escape phase, regulatory DCs sustain tumor ...
Flow cytometry. Cells were stained with fluorochrome-labeled antibodies of interest for 30 min on ice. For intracellular staining, the cells were fixed and permeabilized using a Fix/Permeabilisation buffer (eBiosciences; Cat# 00-8333-56) for 1 h. After washing with PBS, the cells ...
K Vermaelen,R Pauwels - 《Cytometry Part A》 被引量: 451发表: 2004年 Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung The lung hosts multiple populations of macrophages and dendritic cells, which play a crucial role in lung pathology. The accurate identificatio...
Dendritic cells (DCs) develop in the bone marrow from haematopoietic progenitors that have numerous shared characteristics between mice and humans. Human counterparts of mouse DC progenitors have been identified by their shared transcriptional signatures and developmental potential. New findings continue to ...
An alternative flow cytometry strategy for peripheral blood dendritic cell enumeration in the setting of repetitive GM-CSF dosingMedicine(GeneralBackground Enumeration of circulating peripheral blood dendritic cells (DCs) is complicated by the absence of a unique cell surface marker expressed on all DC ...
Cell surface markers of mouse thymic dendritic cells have been studied by flow cytometry after isolation by collagenase digestion, separation of the low-density cell fraction and differential adherence. The dendritic cell preparation had a purity of > 90%, the contaminating population being essentially...
The effects of AEPS on the phagocytic activity, expression of surface molecules, mRNA and protein expression levels of cytokines and chemokines in mouse bone-marrow derived dendritic cells (BMDCs) were determined by flow cytometry, qRT-PCR and ELISA, respectively. The transcriptional profile induced ...
43dNK cells,19,44decidual MHCII+macrophages,42and decidual MHCII+monocytes.42Thus, uterine DCs, in aggregate, are most accurately identified by flow cytometry as cells with a CD11chiMHCII+Ly6Cneg−intF4/80−surface phenotype.CD11b and/or CD103 can then be used to distinguish the two ...
Defining human dendritic cell progenitors by multiparametric flow cytometry. Nat Protoc 2015; 10:1407–1422. Article CAS PubMed PubMed Central Google Scholar Merad M, Sathe P, Helft J, Miller J, Mortha A . The dendritic cell lineage: ontogeny and function of dendritic cells and their ...
Cross-presentation of thymic epithelial cell-associated antigens by thymic DCs is involved in the deletion of autoreactive CD8+ T cells. In the periphery, cross-presentation of self antigens by DCs is also important for the maintenance of tolerance. The manipulation of cross-presentation and in ...