An alternative flow cytometry strategy for peripheral blood dendritic cell enumeration in the setting of repetitive GM-CSF dosingMedicine(GeneralBackground Enumeration of circulating peripheral blood dendritic cells (DCs) is complicated by the absence of a unique cell surface marker expressed on all DC ...
Defining human dendritic cell progenitors by multiparametric flow cytometry. Nat Protoc 2015; 10:1407–1422. Article CAS PubMed PubMed Central Google Scholar Merad M, Sathe P, Helft J, Miller J, Mortha A . The dendritic cell lineage: ontogeny and function of dendritic cells and their ...
Flow cytometry DCs and other cell types express Fc receptors that can non-specifically bind to antibodies. Fc receptor blocking agents should be used in conjunction with antibody-based flow analysis to avoid nonspecific binding. OMIP-044 and OMIP-061 for human and mou...
Cell surface markers of mouse thymic dendritic cells have been studied by flow cytometry after isolation by collagenase digestion, separation of the low-density cell fraction and differential adherence. The dendritic cell preparation had a purity of > 90%, the contaminating population being essentially...
WASp and Arpin deficiencies altered the number of migratory DCs present in skin-draining lymph nodes at steady state by flow cytometry. Conventional skin DCs can be divided into the following two main subtypes based on surface marker expression: type 1 conventional DCs (cDC1s) and type 2 convent...
Cell surface markers of mouse thymic dendritic cells have been studied by flow cytometry after isolation by collagenase digestion, separation of the low-density cell fraction and differential adherence. The dendritic cell preparation had a purity of > 90%, the contaminating population being essentially...
Upon antigen exposure mature antigen-presenting cells are enriched by immunostaining and flow cytometry. Selected cells are further subjected to next generation sequencing for the identification of gRNAs that induce a gain-of-function phenotype. Single CRISPR RNA gene-edited cells are cloned, ...
The WT1/MUC1-DC vaccine was characterized by flow cytometry, and the phenotype showed high levels of migration marker (CCR7), MHC molecules (HLA-ABC and DR), costimulatory molecules (CD80 and CD86), and maturation marker (CD83), all of which are associated with migration of WT1/MUC1-DC ...
(referred to hereafter as CD11bhiDCs).39,40Both subsets derive from blood-borne DC precursors termed pre-DCs.41The uterus is no exception, and CD11bloand CD11bhiDCs can both be visualized in this tissue by flow cytometry.42Critically, these are the only two cell types with LN-homing ...
Flow Cytometry Analysis Double-extracellular/intracellular staining on lung cell suspensions was performed using standard techniques. Briefly, cells were incubated with biotin-coupled mAbs and subsequently with R-phycoerythrin (R-PE)-coupled streptavidin (DAKO). After washes in PBS and permeabilization with...