flow cytometryimmunophenotypeBackground Neoplasms derived from plasmacytoid dendritic cells (PDCs) are currently divided into two broad categories: mature PDC proliferations associated with myeloid neoplasms (MPDMN) and blastic plasmacytoid dendritic cell neoplasm (BPDCN); only BPDCN is recognized in the ...
K Vermaelen,R Pauwels - 《Cytometry Part A》 被引量: 451发表: 2004年 Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung The lung hosts multiple populations of macrophages and dendritic cells, which play a crucial role in lung pathology. The accurate identificatio...
et al. Immune cell dysfunctions in breast cancer patients detected through whole blood multi-parametric flow cytometry assay. Oncoimmunology 5, 1–15 (2016). Article CAS Google Scholar Kirkling, M. E. et al. Notch signaling facilitates in vitro generation of cross-presenting classical dendritic ...
Moreover, in the course of ICD, cancer cells emit a characteristic array of damage-associated molecular patterns (DAMPs), that act as adjuvants on innate pattern recognition receptors (PRRs) expressed by antigen-presenting cells (APCs) of the conventional dendritic cell (DC) type 1 (cDC1) [...
(Fig.1c). Gating strategies used to identify cell populations by flow cytometry in this and subsequent experiments are shown in Supplementary Figs.1–6. Mice that received DCPs had a higher frequency of donor-derived CD45.1+cells among splenocytes than mice that received moDCs or cDC1-like...
Flow cytometry DCs and other cell types express Fc receptors that can non-specifically bind to antibodies. Fc receptor blocking agents should be used in conjunction with antibody-based flow analysis to avoid nonspecific binding. OMIP-044 and OMIP-061 for human and mou...
WANG KH,KEVIN PN,RITA SM,et al.An alternative flow cytometry strategy for peripheral blood dendritic cell enumeration in the setting of repetitive GM-CSF dosing[J].J Translat Med,2006,4:18-32.An alternative flowcytome-trystrategyfor peripheral blood dendritic cell enumeration in the set-ting ...
(L and M) CD8+ T cell densities measured using flow cytometry (L) and IHC staining (M) in tumors from each treatment group in (K). Data were compared using Student’s t test. (N and O) Immunofluorescence analysis of CCR7+TCF-1+CD8+ and GZMB+CD8+ T cells in tumor sections from ...
Anti-type 1 IL-1 receptor (R) mAbs also visualized these receptors by flow cytometry on freshly isolated epidermal dendritic cells. These results provide new evidence that dendritic cells represent a differentiation pathway distinct from lymphocytes and monocytes. Together with recent findings on the ...
Flow cytometry showed polyclonal DC populations after expansion that consisted of CD11c +CD11b + and CD11c CD11b + cells that co-expressed CD40, CD80, CD86, and MHCII. eDCs were functional in mixed lymphocyte reactions and produced tumor necrosis factor-α on phytohemagglutinin stimulation. The ...