14A ((i) complete deglycosylation using a lyophilized master mix (PNGase F-buffer); (ii) complete deglycosylation using lyophilized PNGase F lyophilized buffer);(iii) negative control (no PNGase F)). The analysi
sample by SEC (Supplementary Fig.2; Methods). In the EndoSE235A-Fc complex structure, the Fc region is located at the cleft of the “V”-shaped structure of EndoS, between the GH and the β-sandwich domains. The Fc region is a homodimer of the C-terminal domains of the antibody hea...
the glycan component; no mechanism of protein specificity by this class of enzymes has yet been described. The Asn297-linkedN-glycan is required for IgG binding to Fc γ receptors (FcγRs) and complement C1q, which trigger antibody-mediated effector functions5,6,7,8. The activity of these ...