Stack CM, Dalton JP, Cunneen M, Donnelly S. De-glycosylation of Pichia pastoris-produced Schistosoma mansoni cathepsin B eliminates non-specific reactivity with IgG in normal human serum. J Immunol Methods. 2005;304:151-7.Stack, C. M., J. P. Dalton, M. Cunneen, and S. Donnelly. 2005 ...
In this review de-N-glycosylation was defined as the removal of the glycan(s) from a N-glycosylprotein, by means of enzymes acting on the di-N-acetylchitobiosyl part of the invariant pentasaccharide inner-core of N-glycosylproteins. Peptide-N~(4)-(N-acetyl-β-D-glucosaminyl) asparagine...
Enhancement of the antioxidant activity of orange and lime juices by flavonoid enzymatic de-glycosylationEnhancement of the antioxidant activity of orange and lime juices by flavonoid enzymatic de-glycosylationdoi:10.1016/j.foodres.2013.03.019α
Glycosylation, the attachment of sugar moieties to proteins, is a post-translational modification (PTM) that provides greater proteomic diversity than other PTMs. Glycosylation is critical for a wide range of biological processes, including cell attachment ...
糖基化(Glycosylation)和糖化(Glycation)
Glycosylations were realized with N-allyloxycarbonyl derivatives of D-glucosamine on 4, 6-diols of the gluco and manno series. Depending on the donor used (either a glucosyl bromide or a β-acetate) and on the stoichiometry of reactants, a good regioselectivity could be observed towards...
When 2,3,4,6-tetra--benzyl--glucopyranose, --galactopyranose and 2,3,4-tri--benzyl--xylopyranose were allowed to react in the cold in dichloromethane or acetonitrile as solvent in the presence of trifluoromethanesulfonic (triflic) anhydride, with methyl or benzyl esters of the -(...
Cytosolic peptide:N-glycanase (NGLY1) is a widely conserved enzyme involved in de–N-glycosylation of N-glycosylated proteins. Mutations in the human NGLY1 gene cause global developmental delay and multisystemic symptoms, but the molecular mechanism underlying pathogenesis remains poorly understood. ...
Activities of two de- N -glycosylation enzymes, PNGase (peptide N 4 ( N -acetyl-glucosaminyl)asparagine amidase) and ENGase (endo N -acetyl-尾-D-glucosaminidase), involved in the release of N-glycans from N -glycoproteins, were monitored in several organs of tomato plants ( Lycopersicon ...
The occurrence of two enzymes performing de‐N‐glycosylation of glycoproteins, namely, endo‐ N ‐acetyl‐β‐ d ‐glucosaminidase (ENGase, EC 3.2.1.96) and peptide‐ N4 ‐( N ‐acetyl‐β‐ d ‐glucosaminyl) asparagine amidase (PNGase, EC 3.5.1.52) was investigated in barley, cv. Plais...