A rapid tool for assessing the protein conformation by partial de-glycosylation is also presented wherein the partial de-glycosylated protein is analysed using capillary electrophoresis (CE-SDS).Anushikha THAKURSeija ROHILShrivardhan PATILMeenakshi SUDHAKARAN...
Glycosylation, the attachment of sugar moieties to proteins, is a post-translational modification (PTM) that provides greater proteomic diversity than other PTMs. Glycosylation is critical for a wide range of biological processes, including cell attachment to...
糖基化(Glycosylation)和糖化(Glycation)
Glycosylations were realized with N-allyloxycarbonyl derivatives of D-glucosamine on 4, 6-diols of the gluco and manno series. Depending on the donor used (either a glucosyl bromide or a β-acetate) and on the stoichiometry of reactants, a good regioselectivity could be observed towards...
Enhancement of the antioxidant activity of orange and lime juices by flavonoid enzymatic de-glycosylationEnhancement of the antioxidant activity of orange and lime juices by flavonoid enzymatic de-glycosylationdoi:10.1016/j.foodres.2013.03.019α
Cytosolic peptide:N-glycanase (NGLY1) is a widely conserved enzyme involved in de–N-glycosylation of N-glycosylated proteins. Mutations in the human NGLY1 gene cause global developmental delay and multisystemic symptoms, but the molecular mechanism underlying pathogenesis remains poorly understood. ...
When 2,3,4,6-tetra--benzyl--glucopyranose, --galactopyranose and 2,3,4-tri--benzyl--xylopyranose were allowed to react in the cold in dichloromethane or acetonitrile as solvent in the presence of trifluoromethanesulfonic (triflic) anhydride, with methyl or benzyl esters of the -(...
Activities of two de- N -glycosylation enzymes, PNGase (peptide N 4 ( N -acetyl-glucosaminyl)asparagine amidase) and ENGase (endo N -acetyl-尾-D-glucosaminidase), involved in the release of N-glycans from N -glycoproteins, were monitored in several organs of tomato plants ( Lycopersicon ...
Glycosylation and processing of prepro-α-factor through the yeast secretory pathway Events in the synthesis and processing of prepro-伪-factor have been assessed with the aid of mutants blocked at various stages in the yeast secretory path......
During the last few years, this has particularly been highlighted in genetic diseases called CDG (Congenital Disorders of Glycosylation). Alterations in mechanisms of vesicle formation due to COPII coat component SEC23B deficiency, or in vesicles tethering, caused by defects of the COG complex, but...