A rapid tool for assessing the protein conformation by partial de-glycosylation is also presented wherein the partial de-glycosylated protein is analysed using capillary electrophoresis (CE-SDS).Anushikha THAKURSeija ROHILShrivardhan PATILMeenakshi SUDHAKARAN...
Glycosylation, the attachment of sugar moieties to proteins, is a post-translational modification (PTM) that provides greater proteomic diversity than other PTMs. Glycosylation is critical for a wide range of biological processes, including cell attachment t...
Enhancement of the antioxidant activity of orange and lime juices by flavonoid enzymatic de-glycosylationEnhancement of the antioxidant activity of orange and lime juices by flavonoid enzymatic de-glycosylationdoi:10.1016/j.foodres.2013.03.019α
Glycosylations were realized with N-allyloxycarbonyl derivatives of D-glucosamine on 4, 6-diols of the gluco and manno series. Depending on the donor used (either a glucosyl bromide or a β-acetate) and on the stoichiometry of reactants, a good regioselectivity could be observed towards...
糖基化(Glycosylation)和糖化(Glycation)
When 2,3,4,6-tetra--benzyl--glucopyranose, --galactopyranose and 2,3,4-tri--benzyl--xylopyranose were allowed to react in the cold in dichloromethane or acetonitrile as solvent in the presence of trifluoromethanesulfonic (triflic) anhydride, with methyl or benzyl esters of the -(...
Activities of two de- N -glycosylation enzymes, PNGase (peptide N 4 ( N -acetyl-glucosaminyl)asparagine amidase) and ENGase (endo N -acetyl-尾-D-glucosaminidase), involved in the release of N-glycans from N -glycoproteins, were monitored in several organs of tomato plants ( Lycopersicon ...
Cytosolic peptide:N-glycanase (NGLY1) is a widely conserved enzyme involved in de–N-glycosylation of N-glycosylated proteins. Mutations in the human NGLY1 gene cause global developmental delay and multisystemic symptoms, but the molecular mechanism underlying pathogenesis remains poorly understood. ...
Common de-N-glycosylation protocols usually require a lengthy incubation time. Although pressure cycling technology or scientific microwave reactors can accelerate this enzyme reaction, they may not be easily accessible. In this brief report, we employed an alternative strategy using a standard domestic ...
The occurrence of two enzymes performing de‐N‐glycosylation of glycoproteins, namely, endo‐ N ‐acetyl‐β‐ d ‐glucosaminidase (ENGase, EC 3.2.1.96) and peptide‐ N4 ‐( N ‐acetyl‐β‐ d ‐glucosaminyl) asparagine amidase (PNGase, EC 3.5.1.52) was investigated in barley, cv. Plais...