Ren Wei-fu, Bian Qi. Research advances of protein N-glycosylation’s biological functions and analytical methods and its role in kidney disease[J]. Journal of Clinical Nephrology, 2021, 21(11): 935-941. DOI: 10.3969/j.issn.1671-2390.2021.11.009 ...
Structure of a peptide : N-glycanase-Rad23 complex: Insight into the deglycosylation for denatured glycoproteins. P Natl Acad Sci USA 102, 9144–9149 (2005). 39. Sievers, F. et al. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst ...
2 Identification of the minimal OGA for nanobody-directed deglycosylation on the target protein. a, Enzymatic activities of OGA and its truncations are evaluated on GFP-Nup62. GFP-Nup62 was coexpressed with indicated constructs, enriched by anti-EPEA beads, and analyzed by immunoblotting to ...
TheTatCel7A,ThaCel7A, andTreCel7A enzymes were characterized for comparison of function. The catalytic domain ofTatCel7A was crystallized, and two structures were determined: without ligand and with thio-cellotriose in the active site. Initial hydrolysis of bacterial cellulose was faster withTatCel7...
* The status of glycosylation is important for quality-control The quality-control mechanism involves a remarkable series of glycosylation and deglycosylation reactions that enables correctly folded proteins to be distinguished from misfolded ones. The importance of quality-control a large fraction of all...
First, to increase proteomic coverage of glycoproteins, crosslinked virion samples were deglycosylated using Protein Deglycosylation Mix II (P6044, NEB) under denaturing conditions according to manufacturer instructions. Lysis of virion samples was performed by adding 3 volumes of lysis buffer containing ...
function. InAspergillusas well asTrichoderma,the O-linked sugars are mainly composed of one to threemannoseunits, linear or branched (Pazur et al. 1980;Gunnarsson et al. 1984)attached tothreonineorserineresidues on the linker peptide. In addition, theseoligosaccharidescan contain glucose andgalactose...
Both alpha-N-acetylgalactosaminidase activity and MAF precursor activity of Gc protein in patient bloodstream can serve as diagnostic and prognostic indices... N Yamamoto,VR Naraparaju,SO Asbell - 《Cancer Research》 被引量: 183发表: 1996年 Deglycosylation of Serum Vitamin D3-Binding Protein by...
Deglycosylation analysis with PNGase F verified that it has no N-glycosylation modification sites. Via the reverse transcription-PCR method, it was found to be expressed only in the human liver, and preferentially in normal tissue. In addition, C19orf24 was shown to be a recently evolved gene...
Deglycosylation by Endoglycosidase F/N-Glycosidase F Digestion A total of 2.5 μl of crude membranes were suspended in 20 μl of PNGase buffer (50 mm potassium phosphate buffer, pH 5.5, 35 mm EDTA, 0.02% SDS, 1% 2-mercaptoethanol, protease inhibitors as above) and digested with 0.5–1 un...