Structure of a peptide : N-glycanase-Rad23 complex: Insight into the deglycosylation for denatured glycoproteins. P Natl Acad Sci USA 102, 9144–9149 (2005). 39. Sievers, F. et al. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst ...
TheTatCel7A,ThaCel7A, andTreCel7A enzymes were characterized for comparison of function. The catalytic domain ofTatCel7A was crystallized, and two structures were determined: without ligand and with thio-cellotriose in the active site. Initial hydrolysis of bacterial cellulose was faster withTatCel7...
Deglycosylation of Serum Vitamin D3-binding Protein Leads to Immunosuppression in Cancer Patients Serum vitamin D3-binding protein (Gc protein) can be converted by beta-galactosidase of B cells and sialidase of T cells to a potent macrophage activating ... N Yamamoto,VR Naraparaju,SO Asbell -...
Deglycosylation of glycoproteins with trifluoromethanesulphonic acid: elucidation of molecular structure and function. Protein TransportGlycosylationAmino Acid SequenceStructure-Activity RelationshipThe alteration of proteins by post-translational modifications, including phosphorylation,... ASB Edge - 《Biochemical ...
function [18]. Differences are primarily found in loops and surface regions distant from the active site. However, there are also small variations in the length and sequence of loop regions along the cellulose-binding path that will affect the dynamics of loop regions and the accessibility of ...
Lectin enrichment and deglycosylation in H2O18 The digested peptides were mixed with a lectin solution containing a combination of ConA, WGA, and RCA 120 (Sigma, USA) at ratio of 2:1 (protein to lectin, w/w). The mixtures were then transferred to new YM-30 filter units. After 1 h ...
Rose MC, Voter WA, Sage H, Brown CF, Kaufman B: Effects of deglycosylation on the architecture of ovine submaxillary mucin glycoprotein. J Biol Chem 259:3167–3172, 1984. Google Scholar Schmid K: Preparation and properties of serum and plasma proteins. XXIX. Separation from human plasma of...
* The status of glycosylation is important for quality-control The quality-control mechanism involves a remarkable series of glycosylation and deglycosylation reactions that enables correctly folded proteins to be distinguished from misfolded ones. The importance of quality-control a large fraction of all...
Protein engineering is the design and construction of novel proteins. It is the manipulation of the structures of protein so as to produce desired properties or synthesis of proteins with particular structure and novel function. Protein engineering is a highly promising technique within the frame of ...
Both alpha-N-acetylgalactosaminidase activity and MAF precursor activity of Gc protein in patient bloodstream can serve as diagnostic and prognostic indices... N Yamamoto,VR Naraparaju,SO Asbell - 《Cancer Research》 被引量: 183发表: 1996年 Deglycosylation of Serum Vitamin D3-Binding Protein by...