Deglycosylation Mix Buffer 2B6045SVIAL-201 x 1 ml10 X FetuinP6042SVIAL-201 x 0.05 ml10 mg/ml 特性和用法 贮存溶液 50 mM NaCl 20 mM Tris-HCl 2.6 mM EDTA pH 7.5 @ 25°C 热失活 65°C for 20 minutes 优势和特性 应用特性 蛋白去糖基化混合液 II 包含去除所有N-连接、简单O-连接糖链以及...
Analysis of a Fusion Protein using the Protein Deglycosylation Mix II and Mass Spectrometry Detailed Characterization of Antibody Glycan Structure using the N-Glycan Sequencing Kit 工具& 资源 Web 工具 Glycan Analyzer FAQs & 问题解决指南 FAQs What are Glycosidases and their uses? What is...
Two fold serial dilutions of O-Glycosidase are added to a reaction mixture of 5 mg of neuraminidase digested fetuin with 1X GlycoBuffer 2. The reaction mix is then incubated at 37°C for 1 hour. O-linked disaccharide carbohydrates are determined by the Morgan and Elson Assay (1). 优势...
Why is Fetuin a good control substrate for both O-Glycosidase, PNGase F and the Protein Deglycosylation Mix? What is a typical Fetuin deglycosylation reaction condition? Do detergents inhibit exoglycosidases/endoglycosidases? How much endoglycosidase should I use to deglycosylate Fetuin under native ...
Colorimetric LAMP enables simple, visual detection (pink-to-yellow) of amplification of SARS-CoV-2 nucleic acid Reduce risk of carryover contamination, with UDG and dUTP included in the master mix Assay targets N and E regions of the SARS-CoV-2 genome, for optimized sensitivity and specificity...
Why is Fetuin a good control substrate for both O-Glycosidase, PNGase F and the Protein Deglycosylation Mix? What is a typical Fetuin deglycosylation reaction condition? Do detergents inhibit exoglycosidases/endoglycosidases? How much endoglycosidase should I use to deglycosylate Fetuin under nat...
Enzymatic removal ofN– andO-glycans using PNGase F or the Protein Deglycosylation Mix FAQs & 问题解决指南 FAQs How much exoglycosidase should be used? Do detergents inhibit exoglycosidases/endoglycosidases? Why have the NEB Glycosidase enzymes changed reaction buffers? What are the new reaction buf...
1X GlycoBuffer 2 50 mM Sodium Phosphate (pH 7.5 @ 25°C) 贮存溶液 50 mM NaCl 20 mM Tris-HCl 5 mM EDTA 50% Glycerol pH 7.5 @ 25°C 热失活 75°C for 10 minutes 分子量 实际: 36000 daltons 优势和特性 应用特性 Removal of carbohydrate residues from proteins ...
Why is Fetuin a good control substrate for bothO-Glycosidase, PNGase F and the Protein Deglycosylation Mix? What is a typical Fetuin deglycosylation reaction condition? Do detergents inhibit exoglycosidases/endoglycosidases? How much endoglycosidase should I use to deglycosylate Fetuin under native con...
Enzymatic removal ofN– andO-glycans using PNGase F or the Protein Deglycosylation Mix Glycan Analysis of Murine IgG by Enzymatic Digestion with Endo S and PNGase F Followed by Mass Spectrometric Analysis Remove iT PNGase F Effective Release and Recovery of Neutral and Sialylated N glycans ...