Deglycosylation Mix Buffer 2B6045SVIAL-201 x 1 ml10 X FetuinP6042SVIAL-201 x 0.05 ml10 mg/ml 特性和用法 贮存溶液 50 mM NaCl 20 mM Tris-HCl 2.6 mM EDTA pH 7.5 @ 25°C 热失活 65°C for 20 minutes 优势和特性 应用特性 蛋白去糖基化混合液 II 包含去除所有N-连接、简单O-连接糖链以及...
I tried the Protein Deglycosylation Mix II on my glycoprotein and didn’t see removal of the carbohydrate. What could be the problem? How much Protein Deglycosylation Mix II should I use to remove the carbohydrates under native (non-denaturing) conditions? What is a good control substrate for ...
Analysis of a Fusion Protein using the Protein Deglycosylation Mix II and Mass Spectrometry Detailed Characterization of Antibody Glycan Structure using the N-Glycan Sequencing Kit 工具& 资源 Web 工具 Glycan Analyzer FAQs & 问题解决指南 FAQs What are Glycosidases and their uses? What is...
Why is Fetuin a good control substrate for both O-Glycosidase, PNGase F and the Protein Deglycosylation Mix? What is a typical Fetuin deglycosylation reaction condition? Do detergents inhibit exoglycosidases/endoglycosidases? How much endoglycosidase should I use to deglycosylate Fetuin under native ...
Colorimetric LAMP enables simple, visual detection (pink-to-yellow) of amplification of SARS-CoV-2 nucleic acid Reduce risk of carryover contamination, with UDG and dUTP included in the master mix Assay targets N and E regions of the SARS-CoV-2 genome, for optimized sensitivity and specificity...
Analysis of a Fusion Protein using the Protein Deglycosylation Mix II and Mass Spectrometry Enzymatic Deglycosylation of a Protein Containing Core 1 O-glycans with O-Glycosidase 工具& 资源 FAQs & 问题解决指南 FAQs What are the typical reaction conditions forO-Glycosidase?
Analysis of a Fusion Protein using the Protein Deglycosylation Mix II and Mass Spectrometry Detailed Characterization of Antibody Glycan Structure using the N-Glycan Sequencing Kit Characterization of Glycans from Erbitux®, Rituxan® and Enbrel® using PNGase F (Glycerol-free), Recombinant Rem...
This application note describes the enzymatic removal of glycans from a protein using PNGase F, or the Protein Deglycosylation Mix. Read App Note Ph.D.™ Peptide Display Cloning System Last Updated 七月 2022 Application: Glycobiology and Protein Analysis This application note provides protocols...
1X GlycoBuffer 2 50 mM Sodium Phosphate (pH 7.5 @ 25°C) 贮存溶液 50 mM NaCl 20 mM Tris-HCl 5 mM EDTA 50% Glycerol pH 7.5 @ 25°C 热失活 75°C for 10 minutes 分子量 实际: 36000 daltons 优势和特性 应用特性 Removal of carbohydrate residues from proteins ...
Why is Fetuin a good control substrate for both O-Glycosidase, PNGase F and the Protein Deglycosylation Mix? What is a typical Fetuin deglycosylation reaction condition? Do detergents inhibit exoglycosidases/endoglycosidases? How much endoglycosidase should I use to deglycosylate Fetuin under nat...