DEAD-boxRNAhelicases are important players in eukaryotic andbacterial RNAmetabolism. AhelicasefromStaphylococcus aureuswas recently shown to affect RNA decay, most likely via its interaction with the proposed Gram-positive degradosome. Some, but not all,RNAsare stabilized when the helicase CshA is mu...
(C) Schematic representation of the conserved helicase motifs of DDX3X and DDX5 (red boxes) with the respective functional roles. Then, ATP hydrolysis triggers the release of the displaced RNA strand, restoring the original open conformation with the two RecA-like domains separated [6]. In ...
Crystal structures of the RecA‐like domain 1 of Staphylococcus aureus CshA (SaCshA) and of its complex with AMP are reported; SaCshA shares a highly conserved ATP‐binding mechanism with other DEAD‐box RNA helicases.doi:10.1107/S2053230X1801292XChen, Xiaobao...
2B, left), whereas VEGF 5′-UTR-dependent translation of firefly luciferase is increased 2-fold in normoxic and nearly 4-fold in hypoxic extract compared with the control RNA (Fig. 2B, right). The comparable stability of all four mRNAs isolated from the translation reactions indicates that ...
ATP hydrolysisDEAD-box helicaseRNA decayRNA turnovercrystal structureCshA is a dimeric DEAD-box helicase that cooperates with ribonucleases for mRNA turnover. The molecular mechanism for how a dimeric DEAD-box helicase aids in RNA decay remains unknown. Here, we report the crystal structure and ...
DEAD-box RHs have been implicated in RNA synthesis, modification, cleavage, and degradation, as well as in ribosome biogenesis and translation initiation [4]. Moreover, they are also involved in the ATP-dependent rearrangement of intermolecular and intramolecular RNA structures or remodeling of ribonu...