An Arabidopsis ATP-dependent, DEAD-box RNA helicase loses activity upon IsoAsp formation but is restored by PROTEIN ISOASPARTYL METHYLTRANSFERASEdoi:10.1105/tpc.113.113456Orthodox seeds are capable of withstanding severe dehydration. However, in the dehydrated state, Asn and Asp residues in proteins can...
DEAD-box helicases are composed of two RecA-like domains connected by covalent bonds, forming the core of the helicase, which contains approximately 12 conserved motifs (Fig.1). The RecA-like domain 1 includes ATP binding and hydrolysis motifs (Q motif, motif I, and motif II), RNA binding...
thaliana DEAD-box ATP-dependent RNA helicase 7 (also known as AtRH7; AT5G62190) was previously shown experimentally to be localized to the nucleus (Lorković et al. 1997). This evidence supports our phylogenetic analysis suggesting that GUCT-containing RNA helicases are clearly organelle ...
In the absence of ATP and RNA, these two RecA-like domains of the helicase core move freely and are flexible with respect to each other (open conformation). The collaborative binding of ATP and RNA, stimulates these two domains to form a closed position (closed conformation), to induce ATP...
DEAD-box RNA解旋酶参与RNA转录、前体mRNA剪切、核糖体发生、核质运输、蛋白质翻译、RNA降解等重要的生命活动。 2. The DEAD-box RNA helicases is a big ATP-dependent RNA helicases family, which originates from a wide range of organisms ranging from prokaryotes, including viruses, to eukaryotes such ...
Although a number of ATP-dependent RNA helicases are important for constitutive RNA splicing, no helicases have been implicated in alternative RNA splicing... A Honig,D Auboeuf,MM Parker,... - 《Molecular & Cellular Biology》 被引量: 174发表: 2002年 A DEAD-box protein alone promotes group...
Memberso,theDEADbox,amilyo,RNAhelicasesareknowntobeinvolvedinmostcellularprocessesthatrequiremanipulationo,RNAstructureand,inmanycases,exhibitother,unctionsinadditiontotheirestablishedATP-dependentRNAhelicaseactivities.Theythusplaycriticalrolesincellularmetabolismandinmanycaseshavebeenimplicatedincellularproli,erationand...
Then, ATP hydrolysis triggers the release of the displaced RNA strand, restoring the original open conformation with the two RecA-like domains separated [6]. In contrast to the conserved helicase core, the N- and C-terminal extensions are variable and divergent in length and composition. These...
DEAD box proteins catalyze the ATP-dependent unwinding of double-stranded RNA (dsRNA). In addition, they facilitate protein displacement and remodeling of RNA or RNA/protein complexes. Their hallmark feature is local destabilization of RNA duplexes. Here
RNA helicases from the DEAD-box family are found in almost all organisms and have important roles in RNA metabolism. They are associated with many processes ranging from RNA synthesis to RNA degradation. DEAD-box proteins use the energy from ATP hydrolysis to rearrange inter- or intra-molecular...