DDX3 is a highly conserved member of ATP-dependent DEAD-box RNA helicases with multiple functions in RNA metabolism and cellular signaling. Here, we describe a novel function for DDX3 in regulating the mitochondrial stress response in the parasitic protozoanLeishmania.We show that genetic inactivatio...
Background DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked (DDX3X) is a member of the DEAD-box family of ATP dependent RNA helicase. DEAD-box helicases have multiple functions including RNA splicing, mRNA export, transcriptional and translational regulation, RNA decay, and ribosome...
Here we show that members of the large and highly abundant family of RNA-dependent DEAD-box ATPases (DDXs)3 are regulators of RNA-containing phase-separated organelles in prokaryotes and eukaryotes. Using in vitro reconstitution and in vivo experiments, we demonstrate that DDXs promote phase ...
In the absence of ATP and RNA, these two RecA-like domains of the helicase core move freely and are flexible with respect to each other (open conformation). The collaborative binding of ATP and RNA, stimulates these two domains to form a closed position (closed conformation), to induce ATP...
DEAD-box family proteins are a putative ATP-dependent RNA helicase, which form a widely dispersed family of proteins ranging from viruses to mammals, and are associated with nearly all RNA processes. DEAD-box家族蛋白是一类ATP依赖的RNA解旋酶,广泛存在于从细菌到哺乳动物的许多物种中,参与RNA代谢的几...
transcriptional level. RNA helicases are involved in the formation of cytoplasmic ribonucleoprotein (RNP) granules and RNA silencing. A highly conserved central domain is characteristic of DEAD-box RNA helicases and determines their basic biological activity in ATP-dependent unwinding of short RNA ...
Memberso,theDEADbox,amilyo,RNAhelicasesareknowntobeinvolvedinmostcellularprocessesthatrequiremanipulationo,RNAstructureand,inmanycases,exhibitother,unctionsinadditiontotheirestablishedATP-dependentRNAhelicaseactivities.Theythusplaycriticalrolesincellularmetabolismandinmanycaseshavebeenimplicatedincellularproli,erationand...
A chloroplast-localized DEAD-box RNA helicaseAtRH3 is essential for intron splicing and plays an important role in the growth and stress response in ... Lee,KH,Kang - 《Plant Physiology & Biochemistry》 被引量: 0发表: 2014年 Regulation of Alternative Splicing by the ATP-Dependent DEAD-Box ...
DEAD-box家族蛋白是一个ATP依赖的RNA解旋酶家族,参与RNA的各种代谢过程如RNA二级结构变换,转录起始,线粒体RNA剪接,核糖体和剪接体装配、mRNA降解以及维持mRNA的稳定性等。基于DEAD-box家族蛋白的这些生物学功能,它们在精子的生成过程中可能有很重要的作用,最近的一些研究显示这个家族的一些成员如DDX3,DDX4,DDX25等与...
Then, ATP hydrolysis triggers the release of the displaced RNA strand, restoring the original open conformation with the two RecA-like domains separated [6]. In contrast to the conserved helicase core, the N- and C-terminal extensions are variable and divergent in length and composition. These...