An overview of mass cytometry as a tool for high dimensional single cell characterization How to implement the Maxpar Direct Immune Profiling Assay (MDIPA) kit to provide robust comprehensive immune monitoring Key advantages of the MDIPA kit – including automated data ...
Cytometry by Time-of-Flight and Imaging Mass Cytometry Traditional flowcytometryis limited by use of fluorescent dyes that have overlapping spectra, thus limiting the resolution between dyes and number of antibody markers that can be combined (usually <20). Cytometry by time-of-flight (CyTOF) is...
Mass cytometry is a recently developed method merging time-of-flight ICP-MS with flow cytometry23. Single-cell suspensions are labelled with metal isotope-tagged antibodies or other binding probes. Individual cells are then ionized in an argon plasma followed by time-of-flight mass spectrometry, wh...
CyTOF®, also known as mass cytometry, takes advantage of the low spectral overlap between heavy metal isotopes. In this technique, heavy metals are conjugated to antibodies, and their unique mass signatures are detected by time-of-flight mass spectrometry. Heavy metals take the place of fluorop...
Mass cytometry, also called cytometry by time-of-flight, is an emerging powerful single-cell proteomic analysis technique which utilizes rare metal isotopes instead of fluorophores for antibodies labeling to break the limit of multiplexing capability of FACS (Table 1; Fig. 1A) (Bandura et al., ...
(cytometry by time of flight–CyTOF) with visual interactive stochastic neighbor embedding techniques are currently being developed by us and others, to improve the sensitivity and specificity of flow based techniques to diagnose CMML11. Using this method it has been shown that CD14/16 markers are...
Mass cytometry (or Cytometry by Time Of Flight mass spectrometry, CyTOF) has already been employed in clinical studies to comprehensively survey patients' circulating immune system. As interest in the "bedside" application of mass cytometry is growing, the delineation of relevant methodological issues ...
Nevertheless, the vast data generated by cytometry by time-of-flight (CyTOF) poses a significant analytical challenge. To address this, we describe ImmCellTyper (https://github.com/JingAnyaSun/ImmCellTyper), a novel toolkit for CyTOF data analysis. This framework incorporates BinaryClust, an ...
Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes. ► T cell specificity, phenotype, and function can be assessed by mass spectrometry ► Principal component analysis revealed a common pattern of phe.....
Summary Matrix‐assisted laser desorption ionisation time‐of‐flight mass spectrometry (MALDI‐TOF MS) may soon replace routine electrophoretic methods for monitoring monoclonal proteins in patients with multiple myeloma. To further evaluate the clinical utility of this assay, we compared the performance ...