Masscytometry, also called cytometry by time-of-flight, is an emerging powerful single-cellproteomicanalysis technique which utilizes rare metal isotopes instead of fluorophores forantibodies labelingto break the limit of multiplexing capability of FACS (Table 1;Fig. 1A) (Bandura et al., 2009;Bend...
CyTOF®, also known as mass cytometry, takes advantage of the low spectral overlap between heavy metal isotopes. In this technique, heavy metals are conjugated to antibodies, and their unique mass signatures are detected by time-of-flight mass spectrometry. Heavy metals take the place of fluorop...
Masscytometry, also calledcytometryby time-of-flight, is an emerging powerful single-cellproteomicanalysis technique which utilizes rare metal isotopes instead of fluorophores forantibodies labelingto break the limit of multiplexing capability of FACS (Table 1;Fig. 1A) (Bandura et al., 2009;Bendall...
Article https://doi.org/10.1038/s41467-023-37478-w Comparative analysis of dimension reduc- tion methods for cytometry by time-of- flight data Received: 12 July 2022 Accepted: 20 March 2023 Published online: 01 April 2023 Check for updates Kaiwen Wang 1, Yuqiu Yang 1,2, Fangjiang Wu 2,...
Article https://doi.org/10.1038/s41467-023-37478-w Comparative analysis of dimension reduc- tion methods for cytometry by time-of- flight data Received: 12 July 2022 Accepted: 20 March 2023 Published online: 01 April 2023 Check for updates Kaiwen Wang 1, Yuqiu Yang 1,2, Fangjiang Wu 2,...
cytometry by time-of-flight provides a label-free approach for inorganic nanoparticle quantitation in cells. Furthermore, mass cytometry can enumerate AuNPs with a lower detection limit of∼10 AuNPs (3 nm core size) in a single cell with tandem multiparameter cellular phenotyping. Using the ...
Start by addingsamplemetadata, such as timepoint, donor, dosage,and treatment, using a familiar spreadsheet interface.Then,use that metadata tosystematically gateyour data,automatically lay outyour visualizations, or filter your dataset down toasubset of interest. This approachreduces human error andsav...
Newell EW, Sigal N, Bendall SC, Nolan GP, Davis MM (2012) Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8(+) T cell phenotypes. Immunity 36:142–152 Bendall SC, Simonds EF, Qiu P, Amir el AD, Krutzik PO,...
Waki, M., Ide, Y., Ishizaki I., et al. (2014). Single-cell time-of-flight secondary ion mass spectrometry reveals that human breast cancer stem cells have significantly lower content of palmitoleic acid compared to their counterpart non-stem cancer cells.Biochimie, 107 Pt A, 73–7. ...
Flow Cytometry Year Development 1954 1965 1967 1983 1990 1995 1999 2003 An instrument in which an electronic measurement for cell counting and sizing was made on cells flowing in a conductive liquid with one cell at a time passing a measuring point was first described by Wal...