传统流式细胞技术和质谱流式细胞技术相比,主要有两点不同:1.标签系统的不同,前者主要使用各种荧光基团作为抗体的标签,后者则使用各种金属元素作为标签;2.检测系统的不同,前者使用激光器和光电倍增管作为检测手段,而后者使用ICP质谱技术作为检测手段。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Fli...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
CyTOF®, or mass cytometry, uses molecularly tagged antibodies to detect and quantify specific cellular antigens, allowing for highly multiplexed assays. The SIgN Mass Cytometry facility houses a next-generation mass cytometer that employs a type of mass spectrometry approach known as Inductively Coupl...
By using metal-labeled probes and mass spectrometric analysis (cytometry by time-of-flight, or CyTOF) of human CD8 + Tcells, we analyzed theexpression of many more proteins than previously possible with fluorescent labels, including surface markers, cytokines, and antigen specificity with modified ...
质谱流式细胞术(mass cytometry),是流式细胞技术与质谱分析技术结合在一起,发展出了的新技术,能在单细胞水平上同时分析超过50种细胞参数,极大的增加了流式细胞分析评估复杂细胞系统和过程的能力。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Flight),其原理简单来说是利用质谱原理对单细胞进行多参...
Article https://doi.org/10.1038/s41467-023-37478-w Comparative analysis of dimension reduc- tion methods for cytometry by time-of- flight data Received: 12 July 2022 Accepted: 20 March 2023 Published online: 01 April 2023 Check for updates Kaiwen Wang 1, Yuqiu Yang 1,2, Fangjiang Wu 2,...
CyTOF®, also known as mass cytometry, takes advantage of the low spectral overlap between heavy metal isotopes. In this technique, heavy metals are conjugated to antibodies, and their unique mass signatures are detected by time-of-flight mass spectrometry. Heavy metals take the place of fluorop...
Masscytometryis the fusion of two technologies: flow cytometry and mass spectrometry. The instrument at the heart of this technology is the mass cytometer or CyTOF® (cytometry by time-of-flight). Similarly to flow cytometry,mass cytometryenables multiparametric single-cell analyses, but cells are...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
Recently emerged mass cytometry (cytometry by time﹐f‐flight [CyTOF]) technology permits the identification and quantification of inherently diverse cellular systems, and the simultaneous measurement of functional attributes at the singleヽell resolution. By virtue of its multiplex ability with limited ...