传统流式细胞技术和质谱流式细胞技术相比,主要有两点不同:1.标签系统的不同,前者主要使用各种荧光基团作为抗体的标签,后者则使用各种金属元素作为标签;2.检测系统的不同,前者使用激光器和光电倍增管作为检测手段,而后者使用ICP质谱技术作为检测手段。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Fli...
CyTOF®, or mass cytometry, uses molecularly tagged antibodies to detect and quantify specific cellular antigens, allowing for highly multiplexed assays. The SIgN Mass Cytometry facility houses a next-generation mass cytometer that employs a type of mass spectrometry approach known as Inductively Coupl...
目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Flight),其原理简单来说是利用质谱原理对单细胞进行多参数检测的流式技术,既继承了传统流式细胞仪的高速分析的特点,又具有质谱检测的高分辨能力。 CyTOF2 质谱流式(mass cytometry)可以在单细胞分辨率下可实现精细免疫细胞分群、细胞周期检测及揭示细胞内...
Cytometry by Time-of-Flight Shows Combinato- rial Cytokine Expression and Virus-Specific Cell Niches within a Continuum of CD8(+) T Cell Pheno- types. Immunity. 2012; 36(1):142-52. doi: 10.1016/j.immuni.2012.01.002 PMID: 22265676
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
It's called CyTOF, which stands for cytometry by time-of-flight, and it gives researchers greater insight into the intricacies of immunecellsthan ever before. Established at Yale last year, the CyTOF tool is one of an estimated 90 in use by researchers and pharmaceutical companies around the ...
Bringing immune profiling one step closer to clinics with cytometry by time-of-flight (CyTOF) Share on XShare on FacebookShare on LinkedinSend to a friendRefer a friendFavourite A meaningful understanding of the immune system during the disease course and response ...
TIME-of-flight spectrometryLEUCOCYTESAims Atherosclerosis is characterized by the abundant infiltration of myeloid cells starting at early stages of disease. Myeloid cells are key players in vascular immunity during atherogenesis. However, the subsets of vascular myeloid cells have eluded reso...
基于“Time-of-flight”的质谱流式Helios系统(CyTOF),利用金属元素取代荧光基团作为标记物,可以对数以百万的单细胞进行快速分析检测,这一技术正以其强大的创新能力,为人类健康与疾病的研究带来前所未有的突破,并在肿瘤、免疫、肿瘤免疫治疗等领域成为必不可少的工具之一。