传统流式细胞技术和质谱流式细胞技术相比,主要有两点不同:1.标签系统的不同,前者主要使用各种荧光基团作为抗体的标签,后者则使用各种金属元素作为标签;2.检测系统的不同,前者使用激光器和光电倍增管作为检测手段,而后者使用ICP质谱技术作为检测手段。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Fli...
Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes. Immunity. 36 (1): 142-152, 2012.3.Bendall, S.C., et al. Single-cell mass cytometry of differential immune and drug responses across a human hema...
Cytometry by time-of-flight shows combinatorial cytokine expression and virus- specific cell niches within a continuum of CD8+ T cell phenotypes. Immunity 36: 142-152.Newell EW, Sigal N, Bendall SC, Nolan GP, Davis MM. Cytometry by Time-of-Flight Shows Combinatorial Cytokine Expression and ...
质谱流式细胞术(mass cytometry),是流式细胞技术与质谱分析技术结合在一起,发展出了的新技术,能在单细胞水平上同时分析超过50种细胞参数,极大的增加了流式细胞分析评估复杂细胞系统和过程的能力。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Flight),其原理简单来说是利用质谱原理对单细胞进行多参...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
CyTOF®, also known as mass cytometry, takes advantage of the low spectral overlap between heavy metal isotopes. In this technique, heavy metals are conjugated to antibodies, and their unique mass signatures are detected by time-of-flight mass spectrometry. Heavy metals take the place of fluorop...
Article https://doi.org/10.1038/s41467-023-37478-w Comparative analysis of dimension reduc- tion methods for cytometry by time-of- flight data Received: 12 July 2022 Accepted: 20 March 2023 Published online: 01 April 2023 Check for updates Kaiwen Wang 1, Yuqiu Yang 1,2, Fangjiang Wu 2,...
Mass cytometry, also known as CyTOF (Cytometry by Time-Of-Flight) combines the principles of flow cytometry with mass spectrometry to enable high-dimensional analysis of individual cells. This technology provides unparalleled detail, making it ideal for understanding complex cellular processes and interac...
Masscytometry, also called cytometry by time-of-flight, is an emerging powerful single-cellproteomicanalysis technique which utilizes rare metal isotopes instead of fluorophores forantibodies labelingto break the limit of multiplexing capability of FACS (Table 1;Fig. 1A) (Bandura et al., 2009;Bend...
Masscytometry, also calledcytometryby time-of-flight, is an emerging powerful single-cellproteomicanalysis technique which utilizes rare metal isotopes instead of fluorophores forantibodies labelingto break the limit of multiplexing capability of FACS (Table 1;Fig. 1A) (Bandura et al., 2009;Bendall...