approach and utilizes a time-of-flight mass spectrometer. In mass cytometry, cells are labeled with antibodies tagged with highly purified, stable isotypes and are then quantified using time-of-flight mass spectrometry[119]. It measures the abundance of the metal isotopes conjugated to the ...
Mass cytometry (or Cytometry by Time Of Flight mass spectrometry, CyTOF) has already been employed in clinical studies to comprehensively survey patients' circulating immune system. As interest in the "bedside" application of mass cytometry is growing, the delineation of relevant methodological issues ...
CyTOF®, also known as mass cytometry, takes advantage of the low spectral overlap between heavy metal isotopes. In this technique, heavy metals are conjugated to antibodies, and their unique mass signatures are detected by time-of-flight mass spectrometry. Heavy metals take the place of fluorop...
Mass cytometry is a recently developed method merging time-of-flight ICP-MS with flow cytometry23. Single-cell suspensions are labelled with metal isotope-tagged antibodies or other binding probes. Individual cells are then ionized in an argon plasma followed by time-of-flight mass spectrometry, wh...
Mass cytometry (or Cytometry by Time Of Flight mass spectrometry, CyTOF) has already been employed in clinical studies to comprehensively survey patients' circulating immune system. As interest in the "bedside" application of mass cytometry is growing, the delineation of relevant methodological issues ...
Fig. 1: Flow cytometry and time of flight mass cytometry assessments of peripheral blood samples from patients with myeloid malignancies. aMonocyte subset analysis by flow cytometry on a peripheral blood sample from a patient with chronic myeloid leukemia, demonstrating an expanded classical monocyte/M0...
Nevertheless, the vast data generated by cytometry by time-of-flight (CyTOF) poses a significant analytical challenge. To address this, we describe ImmCellTyper (https://github.com/JingAnyaSun/ImmCellTyper), a novel toolkit for CyTOF data analysis. This framework incorporates BinaryClust, an ...
For the first time, we analyzed ASC speck detection by HCI in PBMCs and demonstrated advantages of this technique, such as high-throughput, algorithm-driven image quantification and 3D-rendering. Thus, inflammasome activation by ASC speck formation can be detected by various technical methods. ...
This is termed time of flight. 10 Macey 6. Amplification Some particles may also be better differentiated if the original peak or integrated pulse is amplified. Normal amplification accentuates the differences between pulses, but in some cases this may not be sufficient to diff...
The recent development of a high resolution laser ablation laser module has brought tissue imaging modality, at 1 μm per pixel resolution, to cytometry by time of flight mass cytometers (CyTOF). This imaging mass cytometry (IMC) technique utilises antibody-conjugated isotopes of rare earth metal...