Cytometry by time-of-flight shows combinatorial cytokine expression and virus- specific cell niches within a continuum of CD8+ T cell phenotypes. Immunity 36: 142-152.Newell EW, Sigal N, Bendall SC, Nolan GP, Davis MM. Cytometry by Time-of-Flight Shows Combinatorial Cytokine Expression and ...
Tissue sections are labeled with metal-tagged antibody using IHC protocols. The tissue is then ablated in a laser ablation chamber and transported by a stream of inert gas into the CyTOF® for analysis. The measured isotope signals are plotted using the coordinates of each laser shot, resultin...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
It's called CyTOF, which stands for cytometry by time-of-flight, and it gives researchers greater insight into the intricacies of immunecellsthan ever before. Established at Yale last year, the CyTOF tool is one of an estimated 90 in use by researchers and pharmaceutical companies around the ...
Masscytometry, also calledcytometryby time-of-flight, is an emerging powerful single-cellproteomicanalysis technique which utilizes rare metal isotopes instead of fluorophores forantibodies labelingto break the limit of multiplexing capability of FACS (Table 1;Fig. 1A) (Bandura et al., 2009;Bendall...
基于“Time-of-flight”的质谱流式Helios系统(CyTOF),利用金属元素取代荧光基团作为标记物,可以对数以百万的单细胞进行快速分析检测,这一技术正以其强大的创新能力,为人类健康与疾病的研究带来前所未有的突破,并在肿瘤、免疫、肿瘤免疫治疗等领域成为必不可少的工具之一。
摘要: Mass cytometry (cytometry by time-of-flight detection [CyTOF]) is a bioanalytical technique that enables the identification and quantification of diverse features of cellular systems with single-ce...收藏 引用 批量引用 报错 分享 全部来源 求助全文 ACS 相似文献...
Mass cytometry, or Cytometry by Time-Of-Flight, is a powerful new platform for high-dimensional single-cell analysis of the immune system. It enables the simultaneous measurement of over 40 markers on individual cells through the use of monoclonal antibodies conjugated to rare-earth heavy-metal is...
Jiang’s not alone in embracing multi-dimensional profiling approaches in single cell experiments. Jonas Schulte-Schrepping from the University of Bonn, Germany, and colleagues recently described how high-parameter flow cytometry, along with scRNA-seq and cytometry by time of flight, could be used...
Mass cytometry, also known as CyTOF (Cytometry by Time-Of-Flight) combines the principles of flow cytometry with mass spectrometry to enable high-dimensional analysis of individual cells. This technology provides unparalleled detail, making it ideal for understanding complex cellular processes and interac...