Article https://doi.org/10.1038/s41467-023-37478-w Comparative analysis of dimension reduc- tion methods for cytometry by time-of- flight data Received: 12 July 2022 Accepted: 20 March 2023 Published online: 01 April 2023 Check for updates Kaiwen Wang 1, Yuqiu Yang 1,2, Fangjiang Wu 2,...
Nevertheless, there is a high level of complementarity between these tools, so the choice of method should depend on the underlying data structure and the analytical needs.#Dimension reduction (DR) is a key step of Cytometry by Time-of-Flight (CyTOF) data analysis. Here, the authors bench...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
Tissue sections are labeled with metal-tagged antibody using IHC protocols. The tissue is then ablated in a laser ablation chamber and transported by a stream of inert gas into the CyTOF® for analysis. The measured isotope signals are plotted using the coordinates of each laser shot, resultin...
Mass cytometry, also called cytometry by time-of-flight, is an emerging powerful single-cell proteomic analysis technique which utilizes rare metal isotopes instead of fluorophores for antibodies labeling to break the limit of multiplexing capability of FACS (Table 1; Fig. 1A) (Bandura et al., ...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
Jiang’s not alone in embracing multi-dimensional profiling approaches in single cell experiments. Jonas Schulte-Schrepping from the University of Bonn, Germany, and colleagues recently described how high-parameter flow cytometry, along with scRNA-seq and cytometry by time of flight, could be used...
Masscytometry, also called cytometry by time-of-flight, is an emerging powerful single-cellproteomicanalysis technique which utilizes rare metal isotopes instead of fluorophores forantibodies labelingto break the limit of multiplexing capability of FACS (Table 1;Fig. 1A) (Bandura et al., 2009;Bend...
CyTOF®, also known as mass cytometry, takes advantage of the low spectral overlap between heavy metal isotopes. In this technique, heavy metals are conjugated to antibodies, and their unique mass signatures are detected by time-of-flight mass spectrometry. Heavy metals take the place of fluorop...
Mass cytometry, also known as CyTOF (Cytometry by Time-Of-Flight) combines the principles of flow cytometry with mass spectrometry to enable high-dimensional analysis of individual cells. This technology provides unparalleled detail, making it ideal for understanding complex cellular processes and interac...