质谱流式(mass cytometry)可以在单细胞分辨率下可实现精细免疫细胞分群、细胞周期检测及揭示细胞内信号转导途径等,应用型J强! 质谱流式细胞技术继承了传统流式细胞仪的高速分析的特点,又具有质谱检测的高分辨能力,是流式细胞技术一个新的发展方向。 传统流式细胞技术和质谱流式细胞技术相比,主要有两点不同:1.标签系...
CyTOF®, or mass cytometry, uses molecularly tagged antibodies to detect and quantify specific cellular antigens, allowing for highly multiplexed assays. The SIgN Mass Cytometry facility houses a next-generation mass cytometer that employs a type of mass spectrometry approach known as Inductively Coupl...
Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF) Mass cytometry has emerged as a new state-of-the-art technology that enables in-depth characterization of cellular populations and functions at a single ce... T Smets,F Stevenaert,H Adam...
To address this, we used cytometry time-of-flight (CyTOF) imaging mass cytometry (IMC) to simultaneously quantify the expression of 35 protein markers, characterizing the microenvironment of 5 benign nevi and 67 melanomas. We profiled more than 220,000 individual cells to identify melanoma, ...
质谱流式细胞术(mass cytometry),是流式细胞技术与质谱分析技术结合在一起,发展出了的新技术,能在单细胞水平上同时分析超过50种细胞参数,极大的增加了流式细胞分析评估复杂细胞系统和过程的能力。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Flight),其原理简单来说是利用质谱原理对单细胞进行多参...