传统流式细胞技术和质谱流式细胞技术相比,主要有两点不同:1.标签系统的不同,前者主要使用各种荧光基团作为抗体的标签,后者则使用各种金属元素作为标签;2.检测系统的不同,前者使用激光器和光电倍增管作为检测手段,而后者使用ICP质谱技术作为检测手段。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Fli...
目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Flight),其原理简单来说是利用质谱原理对单细胞进行多参数检测的流式技术,既继承了传统流式细胞仪的高速分析的特点,又具有质谱检测的高分辨能力。 CyTOF2 质谱流式(mass cytometry)可以在单细胞分辨率下可实现精细免疫细胞分群、细胞周期检测及揭示细胞内...
Tissue sections are labeled with metal-tagged antibody using IHC protocols. The tissue is then ablated in a laser ablation chamber and transported by a stream of inert gas into the CyTOF® for analysis. The measured isotope signals are plotted using the coordinates of each laser shot, resultin...
By using metal-labeled probes and mass spectrometric analysis (cytometry by time-of-flight, or CyTOF) of human CD8 + Tcells, we analyzed theexpression of many more proteins than previously possible with fluorescent labels, including surface markers, cytokines, and antigen specificity with modified ...
It's called CyTOF, which stands for cytometry by time-of-flight, and it gives researchers greater insight into the intricacies of immunecellsthan ever before. Established at Yale last year, the CyTOF tool is one of an estimated 90 in use by researchers and pharmaceutical companies around the ...
基于“Time-of-flight”的质谱流式Helios系统(CyTOF),利用金属元素取代荧光基团作为标记物,可以对数以百万的单细胞进行快速分析检测,这一技术正以其强大的创新能力,为人类健康与疾病的研究带来前所未有的突破,并在肿瘤、免疫、肿瘤免疫治疗等领域成为必不可少的工具之一。
Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF) Mass cytometry has emerged as a new state-of-the-art technology that enables in-depth characterization of cellular populations and functions at a single ce... T Smets,F Stevenaert,H Adam...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
Cytometry by Time-of-Flight and Imaging Mass Cytometry Traditional flowcytometryis limited by use of fluorescent dyes that have overlapping spectra, thus limiting the resolution between dyes and number of antibody markers that can be combined (usually <20). Cytometry by time-of-flight (CyTOF) is...
摘要: Mass cytometry (cytometry by time-of-flight detection [CyTOF]) is a bioanalytical technique that enables the identification and quantification of diverse features of cellular systems with single-ce...收藏 引用 批量引用 报错 分享 全部来源 求助全文 ACS 相似文献...