传统流式细胞技术和质谱流式细胞技术相比,主要有两点不同:1.标签系统的不同,前者主要使用各种荧光基团作为抗体的标签,后者则使用各种金属元素作为标签;2.检测系统的不同,前者使用激光器和光电倍增管作为检测手段,而后者使用ICP质谱技术作为检测手段。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Fli...
目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Flight),其原理简单来说是利用质谱原理对单细胞进行多参数检测的流式技术,既继承了传统流式细胞仪的高速分析的特点,又具有质谱检测的高分辨能力。 CyTOF2 质谱流式(mass cytometry)可以在单细胞分辨率下可实现精细免疫细胞分群、细胞周期检测及揭示细胞内...
CyTOF®, or mass cytometry, uses molecularly tagged antibodies to detect and quantify specific cellular antigens, allowing for highly multiplexed assays. The SIgN Mass Cytometry facility houses a next-generation mass cytometer that employs a type of mass spectrometry approach known as Inductively Coupl...
By using metal-labeled probes and mass spectrometric analysis (cytometry by time-of-flight, or CyTOF) of human CD8 + Tcells, we analyzed theexpression of many more proteins than previously possible with fluorescent labels, including surface markers, cytokines, and antigen specificity with modified ...
Specifically, she's using CyTOF to explore the role of natural killer (NK) cells in the immune response to West Nile virus. "Right now we're looking at NK cells from patients who had very severe West Nile virus and patients who never knew they were sick with West Nile virus, and we'...
Single-cell aortic preparations were probed with a panel of 35 metal-conjugated antibodies using cytometry by time of flight (CyTOF). Clustering of marker expression on live CD45+ cells from the aortas of ApoE/ mice identified 13 broad populations of leucocytes. Monocyte, macrophage,...
Bringing immune profiling one step closer to clinics with cytometry by time-of-flight (CyTOF) Share on XShare on FacebookShare on LinkedinSend to a friendRefer a friendFavourite A meaningful understanding of the immune system during the disease course and response ...
Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF) Mass cytometry has emerged as a new state-of-the-art technology that enables in-depth characterization of cellular populations and functions at a single ce... T Smets,F Stevenaert,H Adam...
Empty CellicpTOF-S2aicpTOF-R [19]icpTOF-2R [20,21]Vitesse [22,23]CyTOF-3 (Helios) [24–26] m/z Range (Th) 6–270 6–270 6–270 6–280 75–209 TOFMS architecture Orthogonal acceleration, Single-pass reflectron Collision/reaction cell Yes Yes Yes Yes No Argon and major ionic ...
While experimental and informatic techniques around single cell sequencing (scRNA-seq) are advanced, research around mass cytometry (CyTOF) data analysis has severely lagged behind. CyTOF data are notably different from scRNA-seq data in many aspects. This calls for the evaluation and development of...