传统流式细胞技术和质谱流式细胞技术相比,主要有两点不同:1.标签系统的不同,前者主要使用各种荧光基团作为抗体的标签,后者则使用各种金属元素作为标签;2.检测系统的不同,前者使用激光器和光电倍增管作为检测手段,而后者使用ICP质谱技术作为检测手段。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Fli...
Tissue sections are labeled with metal-tagged antibody using IHC protocols. The tissue is then ablated in a laser ablation chamber and transported by a stream of inert gas into the CyTOF® for analysis. The measured isotope signals are plotted using the coordinates of each laser shot, resultin...
目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Flight),其原理简单来说是利用质谱原理对单细胞进行多参数检测的流式技术,既继承了传统流式细胞仪的高速分析的特点,又具有质谱检测的高分辨能力。 CyTOF2 质谱流式(mass cytometry)可以在单细胞分辨率下可实现精细免疫细胞分群、细胞周期检测及揭示细胞内...
By using metal-labeled probes and mass spectrometric analysis (cytometry by time-of-flight, or CyTOF) of human CD8 + Tcells, we analyzed theexpression of many more proteins than previously possible with fluorescent labels, including surface markers, cytokines, and antigen specificity with modified ...
01 April 2023 Check for updates Kaiwen Wang 1, Yuqiu Yang 1,2, Fangjiang Wu 2, Bing Song 2, Xinlei Wang 1,3,5,6 & Tao Wang 2,4,6 While experimental and informatic techniques around single cell sequencing (scRNA-seq) are advanced, research around mass cytometry (CyTOF) data analysis ...
(i.e. from ~6Li to238U), whereas the CyTOF instrument—which is designed for antibody cell-labelling experiments—has a truncated mass range from 75 to 209m/z[27]. The icpTOF-S2 and the CyTOF offer similar sensitivities, with detection efficiencies for the most sensitive elements better ...
In-depth CyTOF analysis of the tumor microenvironment identified a higher proportion of activated tissue-resident 纬未 T cells in patients who had prior IO and who benefit from the combination therapy (non-progressors, n=4; progressors, n=5; 6 log fold-change, adjusted P =0.001). We did...
02.35 Time of flight mass cytometry (cytof) and rna sequencing interrogation of ‘pathogenic’ gm-csf lymphocytes in human spondyloarthritisreact-text: 158 Purpose of review: Natural killer (NK) cells, gamma delta (γδ) T-cells and other innate immune cells are important lymphocyte subsets able...
Specifically, she's using CyTOF to explore the role of natural killer (NK) cells in the immune response to West Nile virus. "Right now we're looking at NK cells from patients who had very severe West Nile virus and patients who never knew they were sick with West Nile virus, and we'...
Single-cell aortic preparations were probed with a panel of 35 metal-conjugated antibodies using cytometry by time of flight (CyTOF). Clustering of marker expression on live CD45+ cells from the aortas of ApoE/ mice identified 13 broad populations of leucocytes. Monocyte, macrophage,...