传统流式细胞技术和质谱流式细胞技术相比,主要有两点不同:1.标签系统的不同,前者主要使用各种荧光基团作为抗体的标签,后者则使用各种金属元素作为标签;2.检测系统的不同,前者使用激光器和光电倍增管作为检测手段,而后者使用ICP质谱技术作为检测手段。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Fli...
质谱流式细胞术(mass cytometry),是流式细胞技术与质谱分析技术结合在一起,发展出了的新技术,能在单细胞水平上同时分析超过50种细胞参数,极大的增加了流式细胞分析评估复杂细胞系统和过程的能力。 目前质谱流式细胞技术采用的仪器是CyTOF(Cytometry by Time-Of-Flight),其原理简单来说是利用质谱原理对单细胞进行多参...
2.Newell, E.W., et al. Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes. Immunity. 36 (1): 142-152, 2012. 3.Bendall, S.C., et al. Single-cell mass cytometry of differential immune and dru...
Cytometry by Time-of-Flight Shows Combinato- rial Cytokine Expression and Virus-Specific Cell Niches within a Continuum of CD8(+) T Cell Pheno- types. Immunity. 2012; 36(1):142-52. doi: 10.1016/j.immuni.2012.01.002 PMID: 22265676
TIME-of-flight spectrometryLEUCOCYTESAims Atherosclerosis is characterized by the abundant infiltration of myeloid cells starting at early stages of disease. Myeloid cells are key players in vascular immunity during atherogenesis. However, the subsets of vascular myeloid cells have eluded reso...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
It's called CyTOF, which stands for cytometry by time-of-flight, and it gives researchers greater insight into the intricacies of immunecellsthan ever before. Established at Yale last year, the CyTOF tool is one of an estimated 90 in use by researchers and pharmaceutical companies around the ...
Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes. M. Davis, "Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell ...
Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms offlow cytometryand elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensiti...
Bringing immune profiling one step closer to clinics with cytometry by time-of-flight (CyTOF) Share on XShare on FacebookShare on LinkedinSend to a friendRefer a friendFavourite A meaningful understanding of the immune system during the disease course and response to...