The Cullin-RING ubiquitin ligases (CRLs) make nearly half of all E3s and are major mediators of proteome homeostasis. CRLs are activated by neddylation, a ubiquitin-like modification that is removed by the deneddylase COP9 signalosome (CSN). Here, based on X-ray crystallography and cryoelectron...
研究人员首先检测了CRL依赖的UCEs(ubiquitin-carrying enzymes)对于CRL2s的特异性,发现其中UBE2R2对CRL2s的特异性最强,在这些UCEs中,CRL2s选择性与UBE2R2作用,并且作用范围不只局限于自然底物,也包括拟素化CRL2VHL的新底物。在没有底物或新底物时,许多CRLs会自泛素化,促进其组分中的底物受体降解。于是他们首先用LC...
Cullin-RING ubiquitin ligases (CRLs) comprise the largest known category of ubiquitin ligases. CRLs regulate an extensive number of dynamic cellular processes, including multiple aspects of the cell cycle, transcription, signal transduction, and development. CRLs are multisubunit complexes composed of a...
Cullin-RING ubiquitin ligases (CRLs) comprise the largest known category of ubiquitin ligases. CRLs regulate an extensive number of dynamic cellular processes, including multiple aspects of the cell cycle, transcription, signal transduction, and development. CRLs are multisubunit complexes composed of a ...
E3泛素连接酶参与真核生物的多种生物学过程,许多E3泛素连接酶的活性可以用催化半胱氨酸反应的探针来检测,然而像cullin-RING泛素连接酶(cullin-RING ubiquitin ligases, CRLs)家族缺乏活性位点,而是连接底物和泛素携带酶。 CRLs是一个蛋...
The COP9 signalosome (CSN) is known to bind cullin-RING ubiquitin ligases (CRLs) and to promote their activity in vivo1,2,3. The mechanism of this stimulation has remained enigmatic because CSN's intrinsic and associated enzymatic activities paradoxicall
CuLlin参与泛素复合体(Ubiquitin Complex)的组成,为泛素连接酶(Ubiquitin Ligases E3)提供疏水蛋白骨架,几乎存在于所有真核生物。CuLlin与环蛋白(RING Proteins)形成枯环泛素连接酶复合体(CuLlin-RING Ubiquitin Ligases,CRLs),与泛素等在蛋白质的定位、代谢、功能、调节和降解中都起着十分重要的作用。参与细胞周期、增殖...
The multi-subunit Cullin-really interesting new gene (RING)-ligases (CRLs) comprise the largest ubiquitin E3 subclass. CRLs are protein complexes that include four subunits: a RING H2 finger protein (e.g. Rbx1/Roc1), a member of the cullin family proteins (e.g. Cul1), an adaptor protein...
Cullin-RING ubiquitin ligases (CRLs) represent the largest family of E3 ubiquitin ligases that control most if not all cellular processes. In CUL3-based CRLs, the substrate specificity is conferred by the interaction with one of around 183 existing BTB proteins, implying a broad spectrum of ...
到目前为止被发现的泛素连接酶已有500多种,它主要划分为两大亚家族:第一类是拥有HECT结构域的E3泛素连接酶;另一类则是含有RING结构域的E3泛素连接酶[4,5],由于它们都具有保守的CUL同源结构域,因此又被称为Cullin-RingE3泛素连接酶(cullin-RING-basedE3-ligases,CRLs)。含有RING结构域的E3泛素连接酶是一个庞大的...