The Cullin-RING ligases (CRLs) are the largest family of ubiquitin E3s activated by neddylation and regulated by the deneddylase COP9 signalosome (CSN). The inositol polyphosphate metabolites promote the formation of CRL–CSN complexes, but with unclear mechanism of action. Here, we provide struct...
E3泛素连接酶参与真核生物的多种生物学过程,许多E3泛素连接酶的活性可以用催化半胱氨酸反应的探针来检测,然而像cullin-RING泛素连接酶(cullin-RING ubiquitin ligases, CRLs)家族缺乏活性位点,而是连接底物和泛素携带酶。 CRLs是一个蛋...
但是E3泛素连接酶如何靶向这些底物使其降解的机制仍不清楚。 近日,来自美国内华达大学Gary Kleiger和德国马普所Brenda A. Schulman研究团队在Molecular Cell上发表题为Cullin-RING ligases employ geometrically optimized catalytic partners for substrate targeting的文章,发现泛素携带酶(UCE)促进的催化可能也参与底物选择,UBE...
Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusion 2021, Developmental Cell Citation Excerpt : We focused on E3 ligases, as they control metazoan development and are mutated in distal and nemaline myopathies (Gupta and Beggs, 2014; Rape, 2018). Moreover, tissue-specific ...
Cullin-RING ubiquitin ligases (CRLs) comprise the largest known category of ubiquitin ligases. CRLs regulate an extensive number of dynamic cellular processes, including multiple aspects of the cell cycle, transcription, signal transduction, and development. CRLs are multisubunit complexes composed of a...
Cullin-RING E3 ubiquitin-Ligases (CRLs) are the most prominent class of ubiquitin-ligases. By controlling the stability of a cohort of key regulators, CRLs impinge on many cellular and biological processes such as immunity, development, transcription, cell signalling and cell cycle progression. CRLs...
Structural regulation of cullin-RING ubiquitin ligase complexes Cullin-RING ligases (CRLs) compose the largest class of E3 ubiquitin ligases. CRLs are modular, multisubunit enzymes, comprising interchangeable substrate ... David M Duda and Daniel C Scott and Matthew F Calabrese and Erik S Zimmerman...
Identifying the E3 ubiquitin ligases that regulate NKCC2 expression and activity may provide new targets for the development of specific loop diuretics. Author and article information Journal Journal ID (nlm-ta): Am J Physiol Renal Physiol Journal ID (iso-abbrev): Am J Physiol Renal Physiol...
E3 ubiquitin ligases, particularly those known to be activated in human cancer, become an attractive choice. Cullin-RING Ligases (CRLs) with multiple components are the largest family of E3 ubiquitin ligases and are responsible for ubiquitination of ~20% of cellular proteins degraded through UPS. ...
E3 ubiquitin ligases play essential roles in the degradation and regulation of CKIs during cell cycle progression. During the G2/M phase, the targeting of CKIs for degradation is primarily mediated by the APC/C E3 ubiquitin ligase complex. At other phases of the cell cycle, CRL complexes als...