and cell division.Although the yeast and animal proteasomes are well characterized,there is only limited information on the plant proteasome.We determined the first plant 26S proteasome structure from Spinacia oleracea by single-particle electron cryogenic microscopy at an overall resolution of 3.3 A掳...
Protein homoeostasis in plastids is strategically regulated by the protein quality control system involving multiple chaperones and proteases, among them the Clp protease. Here, we determined the structure of the chloroplast ClpP complex from Chlamydomon
因此,急需一个自动化,高效并且准确从冷冻电镜图像中确定DNA/RNA结构的方法。 2023年10月2日,美国普渡大学Daisuke Kihara研究组 (第一作者为王虓) 在Nature Methods上发表了文章CryoREAD: de novo structure modeling for nucleic acids in cryo-EM maps using deep learning,研究人员 开发了从冷冻电镜图中全自动建模...
Rix7 is an essential type II AAA-ATPase required for the formation of the large ribosomal subunit. Rix7 has been proposed to utilize the power of ATP hydrolysis to drive the removal of assembly factors from pre-60S particles, but the mechanism of release
The cryo–EM structure of AtCRY2W374A was determined to an overall resolution of 2.56 Å, and a tetramer atomic model was built (Figure 1C and Supplemental Figures 2 and 3; Supplemental Table 1), which was much improved compared with previous reported structures (Ma et al., 2020a; Shao...
Cryo-EM structures of the plant plastid-encoded RNA polymerase The cryo-EM structures ofNicotiana tabacum(tobacco) chloroplast RNA polymerase apoenzyme and transcription elongation complexes reveal the composition, ass... XX Wu,WH Mu,F Li,... - 《Cell》 被引量: 0发表: 2024年 Localization of ...
of nucleocapsids of negative-strand RNAviruses, in particular via the exchange subdomains of the nucleoprotein.The structure reveals the mode of the nucleoprotein-RNA interaction and explainswhyeachnucleoprotein of measlesvirus bindssixnucleotides,whereastherespiratory syncytial virus nucleoprotein binds seven....
The structure of a 12-segmented dsRNA reovirus: New insights into capsid stabilization and organization Infecting a wide range of hosts, members of Reovirales (formerly Reoviridae) consist of a genome with different numbers of segmented double stranded RNAs (... Q Zhang,Y Gao,M Baker,... -...
Tobacco curly shoot virus, a monopartite begomovirus associated with betasatellite, causes serious leaf curl diseases on tomato and tobacco in China. Using single-particle cryo-electron microscopy, we determined the structure of tobacco curly shoot virus
ion channel; cryo-EM; protein structure; transmembrane protein; electrophysiology 1. Introduction Ion channels are transmembrane protein complexes with internal pathways for ions to diffuse down their electrochemical gradients rapidly and passively. The opening and closing (gating) of the ion-conducting ...