The experiments, involving eighteen experimentally derived CryoEM images, show that twelve cases have error within 2 . CONCLUSIONS: The tests using both simulated and experimentally derived images show that it is possible for our proposed method to estimate the loop length along the skeleton if the...
CryoDRGN: reconstruction of heterogeneous cryo-EM structures using neural networkswww.nature.com/articles/s41592-020-01049-4 Inferring a Continuous Distribution of Atom Coordinates from Cryo-EM Images using VAEsarxiv.org/abs/2106.14108发布...
Single particle cryo-electron microscopy (cryo-EM) uses the particles extracted from thousands of images to reconstruct 3D structures of macromolecules. The yield of an acquisition session at the microscope depends on the time needed to set up the run and the speed of the acquisition itself....
With cryo-EM, researchers rapidly vitrify a cell, virus, molecular complex, or other structure to preserve samples in their natural state. Scientists use cryo-EM to produce higher-resolution images compared to other structural techniques because of modern electron detectors and image-processing. The ...
schematic diagrams of the wicking process, during which excess solution is wicked away, leaving behind a thin liquid layer on the UFG (f) and rough graphene (g). Right: cryo-EM images showing the uniform vitreous ice on the UFG (f) and wavy vitreous ice on the rough graphene (g). Sca...
This method encodes structures in Fourier space using coordinate-based deep neural networks, and trains these networks from unlabeled 2D cryo-EM images by combining exact inference over image orientation with variational inference for structural heterogeneity. We demonstrate that the proposed method, ...
CryoSegNet is a method using foundational image segmentation model for picking protein particles in cryo-EM micrographs. It is trained on 22 different protein types including membrane protein, signaling protein, transport protein, viral protein, ribosomes, etc. It uses U-Net and SAM's automatic ma...
Structure determination by cryo electron microscopy (cryo-EM) provides information on structural heterogeneity and ensembles at atomic resolution. To obtain cryo-EM images of macromolecules, the samples are first rapidly cooled down to cryogenic temperat
A graph theory method for determination of cryo-EM image focuses Accurate determination of micrograph focuses is essential for averaging multiple images to reach high-resolution 3-D reconstructions in electron cryo-micro... W Jiang,G Fei,Z Liu - 《Journal of Structural Biology》 被引量: 47发表...
With the introduction ofThermo Scientific Smart EPU Software, both automated screening and data acquisition for single particle cryo-EM analysis are now available through an approachable graphical user interface. Featuring unique AI algorithms, Smart EPU Software allows for thousands of images to be coll...