In chapter 3, we apply LUD based algorithms to improve the accuracy of orientation determination from cryo-EM images. In chapter 4, we propose a fast and accurate Fourier-based iterative 3D inversion algorithm in cryo-EM, which exploits the Toeplitz structure of the composition of backward and ...
Single particle analysis cryo EM software for automated cryo electron microscopy data acquisition, enabling high-resolution 3D protein structure determination.
Single particle reconstruction (SPR) from cryo-electron microscopy (EM) is a technique in which the 3D structure of a molecule needs to be determined from its contrast transfer function (CTF) affected, noisy 2D projection images taken at unknown viewing directions. One of the main challenges in...
With cryo-EM, researchers rapidly vitrify a cell, virus, molecular complex, or other structure to preserve samples in their natural state. Scientists use cryo-EM to produce higher-resolution images compared to other structural techniques because of modern electron detectors and image-processing. The ...
Frealign: An Exploratory Tool for Single-Particle Cryo-EM. Frealign is a software tool designed to process electron microscope images of single molecules and complexes to obtain reconstructions at the highest possi... N Grigorieff - 《Other》 被引量: 0发表: 2016年 Gctf: Real-time CTF determina...
schematic diagrams of the wicking process, during which excess solution is wicked away, leaving behind a thin liquid layer on the UFG (f) and rough graphene (g). Right: cryo-EM images showing the uniform vitreous ice on the UFG (f) and wavy vitreous ice on the rough graphene (g). Sca...
Cryo-electron microscopy (cryo-EM) is an experimental technique that captures 2D images of biological molecules and assemblies (protein particles, virus, etc.) at cryogenic temperature using ‘direct’ electron-detection camera technology1. With the advent of cryo-EM, there has been a boom in str...
Secondly, the cryo light microscope’s greatest potential for greater workflow efficiency is the ability to target the structure of interest much more precisely before the time and cost intensive CryoEM preparations begin. Leica’s cryo light microscopy solutions enable image and coordinate export...
With the introduction ofThermo Scientific Smart EPU Software, both automated screening and data acquisition for single particle cryo-EM analysis are now available through an approachable graphical user interface. Featuring unique AI algorithms, Smart EPU Software allows for thousands of images to be coll...
CryoSegNet is a method using foundational image segmentation model for picking protein particles in cryo-EM micrographs. It is trained on 22 different protein types including membrane protein, signaling protein, transport protein, viral protein, ribosomes, etc. It uses U-Net and SAM's automatic ma...