Potter, A relational database for cryoEM: experience at one year and 50,000 images, J. Struct. Biol. 137 (2002) 273 - 282.Fellmann D, Pulokas J, Milligan RA, Carragher B, Potter CS. A relational database for cryoEM: experience at one year and 50 000 images. J Struct Biol. Mar...
笔记:cryosPArc: algorithms for rapid unsupervised cryo-em structure determination 站(http://www.cryosparc.com)可供查阅 Research Objective单粒子低温冷冻电镜是确定生物大分子结构的一种方法,可是显微镜拍到的图片仅仅只是同一个物体(如蛋白质)在各个...一作者使用SGD随机梯度下降法进行三维重建,得到了一个初步的...
Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical technique that can be used to determine the structure of biological macromolecules and assemblies. Historically, its potential for application in drug discovery has been heavily limited by two issues: the minimum ...
Many crystallographic and cryogenic electron microscopy (cryo-EM) structures are available for different enterovirus or other picornavirus capsids under different conditions [see the VIPER database, https://viperdb.org/32]. However, the detailed structure and organization of the encapsidated genomic ...
https://www.youtube.com/watch?v=Asp2Sg-AN-gWe show how to start building an atomic model into a cryoEM map using an atomic structure from the AlphaFold database. We fit the atomic model into the map a, 视频播放量 62、弹幕量 0、点赞数 5、投硬币枚数 1、收
Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical technique that can be used to determine the structure of biological macromolecules and assemblies. Historically, its potential for application in drug discovery has been heavily limited by two issues: the minimum ...
c Relative abundance of Morphologies-1, 2, and others in the cryo-EM data set (n = 200). Full size image Using cryo-EM, we have investigated a sample of TDP-43-derived amyloid fibrils that were formed in vitro from full-length TDP-43. We find that the protein is able to form...
Microsoft Azure & AMD Solution Channel The pasta puzzle: Decoding durum wheat’s DNA for a sustainable future An Italy that can’t grow its own wheat for pasta? It’s a race against time. Using a shared database in a Microsoft super‑computer, scientists from Italy and other countries ar...
Data retrieved from the MemProtMD database and kindly provided by Phillip Stansfeld1 [16]. (b) Trends of the membrane protein reconstructions better than 5 Å resolution by cryo-EM at 200 keV. Annual numbers are indicated and represented as bars. Thumbnails of membrane protein reconstructions...
AM:What is your outlook on cryo-EM and electron microscopy as a way to combat infectious diseases? Dr. Saphire:Going forward, we’re going to need a range of tools. We’re going to continue to need x-ray crystallography for the high-quality data, the reproducibility, and the eas...