CryoEM Processing|CryoEM 数据处理-transfer cryoSPARC single particle to RELION 冠貌 单颗粒冷冻电镜数据处理中用的比较多的软件是RELION和CryoSPARC。两者各有利弊,且不同的人有不同的使用倾向。笔者认为,RELION的MotionCorr和Polish较好,而CryoSPARC的2D,Nu-refinement则较优。对于有些比较难处理的数据集,有些人...
CryoSPARC Live™ is an extension of the CryoSPARC platform that enables real-time cryo-EM data quality assessment and decision making during live data collection, as well as an expedited, streamlined workflow for processing already available data. ...
Cryo-EM processing at the pace of medicinal chemistry on AWS Read more Instrumentation and resources for the preparation of single particle cryo-EM samples Download datasheet Cryo-EM trends for drug discovery Find a selection of case studies supported by cryo-EM Antibody ...
Cryo-EMRELIONImage processingMotion correctionRecent advances in direct electron detectors and improved CMOS cameras have been accompanied by the development of a range of software to take advantage of the data they produce. In particular they allow for the correction of two types of motion in cryo...
Single-particle electron cryomicroscopy (cryo-EM) is a powerful method for determining the structures of biological macromolecules. With automated microscopes, cryo-EM data can often be obtained in a few days. However, processing cryo-EM... A Punjani,JL Rubinstein,DJ Fleet,... - 《Nature Metho...
Cryo-electron microscopy (cryo-EM) of biological molecules in single-particle (i.e., unordered, nonaggregated) form is a new approach to the study of molec... Frank,Joachim - 《Annual Review of Biophysics & Biomolecular Structure》 被引量: 498发表: 2002年 The Condor distributed processing sy...
Built upon the PyTorch version of cryo-EM data processing utilities provided by cryoDRGN 1.0, OPUS-DSD introduces a set of methodological improvements aiming to effectively and reliably capture structural heterogeneities. One critical component of OPUS-DSD is the 3D convolutional neural network with tra...
cryo-em cryoem scipion Updated Feb 21, 2025 C++ KudryashevLab / TomoBEAR Star 28 Code Issues Pull requests Discussions TomoBEAR is a configurable and customizable modular pipeline for streamlined processing of cryo-electron tomographic data for subtomogram averaging. processing template-matching ...
Single particle analysis cryo-EM data acquisition software Single particle cryo-electron microscopy (cryo-EM) uses the particles extracted from thousands of images to reconstruct 3D structures of macromolecules. The yield of an acquisition session at the microscope depends on the time needed to ...
Data are shown as means ± SEM (n = 3 independent experiments). Full size image A pool of p97 expressed in human HEK293 cells also existed as DH, as revealed by negative stain EM and cryo-EM (Supplementary Fig. S4b–d). Affinity capture of endogenous human p97 with small-...