CryoSPARC Live™ is an extension of the CryoSPARC platform that enables real-time cryo-EM data quality assessment and decision making during live data collection, as well as an expedited, streamlined workflow for processing already available data. ...
CryoSPARC Live™ is an extension of the CryoSPARC platform that enables real-time cryo-EM data quality assessment and decision making during live data collection, as well as an expedited, streamlined workflow for processing already available data. ...
Automatic data collection was performed using SerialEM v.3.8.6 software. Cryo-EM data processing workflow is shown in Fig. 1b. Motion correction and contrast transfer function estimation were performed using MotionCorr228 and CTFFIND-4.1.82729, respectively. Particles were first manually picked for...
Researchers at the University of Utah give a great overview from sample purification, grid prepration, screen and evaluation to data collection and image processing. Their website, cryoem101.org, contains chapters for the different steps in the workflow. The text is illustrated with videos and ...
Learn how an integrated structural biology workflow can be used to get crucial structural insights on key protein targets that are of therapeutic interest. 0:00 Ask the Experts: Routine sub-2.5 Å cryo-EM structure determination of GPCRs for drug design Topic: Routine sub-2.5 Å cryo-...
Cryo-EM University Full single particle cryo-EM course alternating theory and practical demonstration videos covering all aspects from sample preparation, microscope design, cameras, to data acquisition. Start EM University now Live virtual webinars ...
Cryo-EM data processing The workflow of the Cryo-EM data processing is shown in Supplementary Fig. 1. All movies were imported into Relion (v 4.0.0)38, followed by Relion’s own motion correction and CTF estimation (CTFFIND, v 4.1.13)39. 3 M particles in total were picked by a tr...
Workflow for single-particle cryo-EM Step 1: Purify To study molecules by single-particle cryo-EM, a specimen must be purified and structurally intact to produce high-quality 3D reconstructions. Ideally, you need to maintain the specimen in a buffer solution that keeps it biochemically active. ...
Cryo-EM data collection statistics are summarized in Table 1. Cryo-EM data processing, model building and refinement Cryo-EM data were processed using cryoSPARC v4.5.130, following the workflow detailed in Supplementary Fig. 1. A total of 15,337 dose-fractionated movies were motion-corrected ...
Cryo-EM structure determination The enhanced ice coverage and the abundance of monodisperse fibril filaments observed with HEPES buffer facilitated the determination of fibril structures formed at physiological pH. The cryo-EM data processing workflow is shown in detail in Fig. S1, and the final struc...