强基因抑制效果:使用dCas9-KRAB CRISPRi系统进行转录抑制通常可以获得高水平的基因抑制效果。 更多适用的基因种类:由于dCas9-KRAB CRISPRi系统是在DNA水平抑制基因表达,因此适用于多种转录本,包括mRNA、非编码RNA、microRNA、反义转录本、核定位RNA以及聚合酶III 转录本的转录抑制。 特异性:dCas9-KRAB CRISPRi系统可...
The “Block and Lock” methodology aims to control HIV-1 latency reactivation, promoting a functional cure. We utilized the CRISPR/dCas9-KRAB platform, which was initially developed to suppress cellular genes transcription, to block drug-induced HIV-1 reactivation in latently infected T cells and ...
Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi 来自 dx.doi.org 喜欢 0 阅读量: 8 作者:FC Edenhofer,A Térmeg,M Ohnuki,J Jocher,Z Kliesmete,E Briem,I Hellmann,W Enard 摘要: Molecular biology ...
CRISPR-dCas9-KRABProstate cancerPSAGene therapyPSA is a type of proto-oncogene that is specifically and highly expressed in embryonic and prostate cancer cells, but not expressed in normal prostate tissue cells. The specific expression of prostate-specific antigen (PSA) is found to be related ...
We utilized the CRISPR/dCas9-KRAB platform, which was initially developed to suppress cellular genes transcription, to block drug-induced HIV-1 reactivation in latently infected T cells and myeloid cells.We identified a set of five sgRNAs targeting the HIV-1 proviral genome (LTR1-LTR5), ...
Selective combination of S100b-specific single-guide RNAs and the dCas9-KRAB fusion significantly suppressed expression of S100b in murine B16 melanoma cells without noticeable off-target effects. S100b suppression resulted in recovery of intracellular WT-p53 and p21 levels and concomitant induction of...