一个完整的dCas9-KRAB CRISPRi慢病毒载体系统包含两个部分,gRNA表达载体和dCas9-KRAB(或者dCas9-KRAB-MeCP2)表达载体。设计基因抑制实验时,只需要设计打靶目的基因的gRNA表达载体即可。 图7. dCas9-KRAB CRISPRi慢病毒载体系统 优势 不改变内源基因组背景:与CRISPR基因编辑、传统基因敲除技术不同,dCas9-KRAB CRIS...
将CAG-LSL-2xKRAB-dCas9-IRES-EGFP-WPRE-pA插入到小鼠Rosa26基因中,建立CRISPRi基因表达调控工具小鼠。该小鼠已去掉stop。 CRISPRi是基于一个没有核酸酶活性的dCas9蛋白,当dCas9被引导到某个基因的转录起始位点TSS(transcription start site)时,dCas9能够物理性阻碍RNA聚合酶的通过,导致基因沉默。为了进一步提高转录...
HH-CAS-197 Lenti-iCas9-neo(Plasmid #85400) HH-YGE-025 pET-NLS-Cas9-6xHis-EGFP pTre3g-CasRxN-tet3G-puro-NC pTre3g-CasRxN-tet3G-puro-NC HH-CAS-195 pTre3g-CasRxN-tet3G-puro HH-CAS-194 pX333-Puro CRISPR双靶点基因编辑质粒 CAS9 双sgRNA嘌呤霉素 HH-CAS-193 pMSCV-LTR-dCas9-VP64-...
HH-ZW-028pYLCRISPR_Cas9Pubi-H(Plasmid #66187)植物基因编辑载体 HH-YGE-012pKD4 细菌表达;基因敲除系统 HH-ZW-024pCBC-MT1T2-(Plasmid#50593) 植物基因编辑质粒 sgRNA表达质粒 HH-YGE-005pC001-huLshC2C2-MBP RNA基因编辑Cas13a Plasmid #79150 HH-CAS-055pET302-6His-dCas9-Halo #72269 原核表达d...
载体类型: 慢病毒,CRISPR 筛选标记: 嘌呤霉素 高拷贝/低拷贝: 高拷贝 生长条件: 37 ℃ 插入基因信息 插入基因名称: humanized dCas9-KRAB T2A Puro 物种来源: S. Pyogenes 插入大小: -- 基因突变: D10A and H840A GenBank编号 -- 启动子 标签/融合蛋白: Flag(插入N端子) 克隆方法: -- 5' 克隆位点...
Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi 来自 dx.doi.org 喜欢 0 阅读量: 8 作者:FC Edenhofer,A Térmeg,M Ohnuki,J Jocher,Z Kliesmete,E Briem,I Hellmann,W Enard 摘要: Molecular biology ...
The observed CRISPRi effect of dCas9 without an effector domain was also of transient nature. Long-term repression of the Snurf gene by KRAB-dCas9 required co-expression of both D3A-dCas9 and D3L (twofold; Fig. 2b). On the other hand, long-term repression of the Snurf gene by Ezh2-d...
The observed CRISPRi effect of dCas9 without an effector domain was also of transient nature. Long-term repression of the Snurf gene by KRAB-dCas9 required co-expression of both D3A-dCas9 and D3L (twofold; Fig. 2b). On the other hand, long-term repression of the Snurf gene by Ezh2-d...
HH-CAS-143pX333 CRISPR双靶点基因编辑质粒 CAS9 双sgRNA HH-CAS-142pAAVS1-NC-CRISPRi (Gen3)73499 HH-CAS-142 HH-CAS-141pAAV-EFS-CjCas9-eGFP-HIF1a 137929 HH-CAS-141 HH-CAS-140pXR003 CasRx gRNA cloning backbone (Plasmid #109053) HH-CAS-139pFC334(Plasmid #87846) HH-CAS-139 HH-CAS-...
HH-ZW-028 pYLCRISPR_Cas9Pubi-H(Plasmid #66187)植物基因编辑载体 HH-YGE-012 pKD4 细菌表达;基因敲除系统 HH-ZW-024 pCBC-MT1T2-(Plasmid#50593) 植物基因编辑质粒 sgRNA表达质粒 HH-YGE-005 pC001-huLshC2C2-MBP RNA基因编辑Cas13a Plasmid #79150 HH-CAS-055 pET302-6His-dCas9-Halo #72269 原...