In this study, we examined the transcriptional activity of the PSA promoter in different prostate cancer cells and normal prostate epithelial cells and determined that it is indeed a prostate cancer cell-specific promoter.Then we constructed the CRISPR-dCas9-KRAB system driven by the PSA promoter,...
(simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 565..4668 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks ...
We also tested RNA-mediated recruitment of epigenetic repressor domains to increase the combinatorial capacity of the epi-dCas9 system while maintaining a reduced number of gRNAs. However, recruitment of MCP-Ezh2 by MS2 stem loop-containing gRNAs did not result in repression of the target locus. ...
box Several tools have been developed to repress or activate gene expression or modulate epigenetic marks at a defined locus. These epigenetic editing tools consist of a DNA binding module and an epigenetic effector domain. The easily programmable CRISPR/Cas9 system is the method of choice for prec...
In the past decade, the development of two innovative technologies, namely, induced pluripotent stem cells (iPSCs) and the CRISPR Cas9 system, has enabled ... G Peter,H Xu,H Akitsu - 《Stem Cells International》 被引量: 13发表: 2017年 ...
Krishna Mohan ParsiErica HennessyNicola KearnsRené MaehrSpringer New YorkParsi KM, Hennessy E, Kearns N, et al. Using an inducible CRISPR-dCas9-KRAB effector system to dissect transcriptional regulation in human embryonic stem cells[J]. Methods Mol Biol, 2017, 1507 :221-233....