Cell line stabilityDNA repairGenetic instabilityMetabolomicsRNA-sequencingTranscriptomicsCell line instability can pose a challenge from both regulatory and business needs perspectives. The regulatory guidelines of International Conference on Harmonization require manufacturers to evaluate the cell substrate with ...
Quality of products, genetic stability, biosimilarity Q Are CHO-MK cells genetically stable? Q Do you perform biosimilarity or immunogenicity analysis? Adventitious agent testing Q Did you perform safety testing for viral and other microorganism contamination of CHO-MK cells? Price, license, and...
To investigate production stability in CHO cells, we analyzed primary clones and their respective subclones. Cell lines derived from single cell progenitors grow into populations of cells with phenotypic heterogeneity. Here I present the genetic and epigenetic characterization of these heterogeneous cell ...
Lessons from the hamster: Cricetulus griseus tissue and CHO cell line proteome comparison. J. Proteome Res. 16:3672-3687(2017) 2. Wurm F.M., Wurm M.J. Cloning of CHO cells, productivity and genetic stability -- a discussion. Processes 5:20.1-20.13(2017) 3. Klanert G., Jadhav V., ...
cell lines should be assessed for stability of the modalchromosome numberand theabsenceof mycoplasmal contamination, purified by cloning, characterized, and stored for later use. Once test methods have been optimized and standardized, the cell-cycle time of all cell types used in the test should ...
The established cell line derived many years ago from Chinese hamster ovary (CHO cells) has been studied for the extent of chromosomal variation. Because this cell line is used extensively for genetic studies, the contribution of chromosome variability to genetic variability has also been examined. ...
and clones were generated by FACS. Clones were analyzedfor growth performance and productconcentration via fed-batch studies andgenetic stability was evaluated during nine-week stability studies, including stabilityfed-batches (initiated at t = 2 weeks, t = 5 weeks, and t = 9 weeks, respectively...
Each chromosome showed a distinct signature of CNVs giving for the first time an insight into CHO chromosomal genome stability from next-generation sequencing data. In the future this method could be used in combination with FISH to validate hypotheses on e.g. the range of genetic reductions and...
This comparative analysis revealed that control of qMab involved both genetic construct and cell line-specific factors. With respect to the former, all cell lines exhibited excess production of light chain (LC) mRNA and polypeptide relative to heavy chain (HC) mediated by more rapid LC ...
Jeggo, P.A. (1985), Genetic analysis of X-ray-sensitive mutants of the CHO cell line, Mutat. Res. 146 265-270.Genetic analysis of X-ray sensitive mutants of the CHO cell line. Mutat. Res - Jeggo - 1985 () Citation Context ... isolated in a single mutant hunt using the CHO-K1 ...