Cell Sciences and TechnologyThe Korean Society for Biotechnology and BioengineeringBiotechnology & Bioprocess EngineeringDu ZM, Mujacic M, Le K, Caspary G, Nunn H, et al. (2013) Analysis of heterogeneity and instability of stable mAb-expressing CHO cells . Biotechnology and Bioprocess Engineering ...
In this work, stable CHO DG44 cell lines expressing a human anti-D Mab were created and single-cell clones were amplified to obtain a series of cultures with varying production rates. During the course of amplification, changes in the Mab gene copy numbers, transcriptional levels of Mab mRNAs...
To create stable cell lines, CHO DG44cells were transfected by electroporationand cultivated under selective conditionsfor three weeks followed by an amplification step with 30 nM MTX for an additional three weeks. Stable individual cell pools were then expanded and clones were generated by FACS. Clo...
“Although only three stable MAbs produced by CHO cell cultures are used in this study,” the team wrote, “the basic principles of the non-perfusionn– 1 seed strategies for shortening seed train and production culture duration or improving titer should be applicable to othe...
SCRAJ-STT216Raji/Human CD19 Knockout Stable Cell Line Development Service FABm004-02APC-Labeled Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a (FMC63) CD9-M52H8HEK293Mouse CD19 Protein, His Tag CD9-M5257HEK293Mouse CD19 Protein, Fc Tag (MALS verified) ...
2024, Chinese Journal of Modern Applied Pharmacy Stability of Protein Pharmaceuticals: Recent Advances 2024, Pharmaceutical Research Strategy for Developing a Stable CHO Cell Line that Produces Large Titers of Trastuzumab Antibody 2023, Frontiers in Bioscience - Elite View all citing articles on Scopus ...
There are few studies defining CHO host cell proteins (HCPs) and the flux of these throughout a downstream purification process. Here we have applied quantitative iTRAQ proteomics to follow the HCP profile of an antibody (mAb) producing CHO-S cell line throughout a standard downstream purification...
Toripalimab-tpzi is a programmed death receptor-1 (PD-1) blocking antibody. Toripalimab-tpzi is a humanizedimmunoglobulinG4 (IgG4) kappa immunoglobulin that has a predicted molecular weight of approximately 150 kDa. It is expressed in arecombinantChinese Hamster Ovary (CHO) mammalian cell expre...
Heavy chain and light chain expression plasmids were transiently cotransfected in ExpiCHO-S™ cells (Thermo Fisher) by using the ExpiFectamine CHO transfection kit (Gibco). The supernatants were harvested after 14 days of culture and the MAbs were purified using protein G agarose resin 4FF (...
Anti-CD47 (magrolimab biosimilar;IgG1) mAb SKU: BME100050 Target: CD47 Application: ELISA; Flow Cyt Price: 50μg $82.00 ; 100 μg $162.00 Overexpression Stable Cell Line Hu_CD47 CHO-S Cell Line SKU: CEL100040 Target: CD47 Application: FACS Data Price:Inquiry Phone...