mRNA stability is very crucial for optimum expression of a protein in any host cell. Several factors such as mRNA secondary structure, 5′ and 3′ untranslated region (UTR) sequences, mRNA length, 5′ Cap, 3′ polyA tail, microRNA (miRNA), codon usage and many other factors determine the...
A stably transfected cell line used for screening of compounds for treating disease interest comprising: (i) An expression system reporter gene DNA consists of a reporter gene encoding a first protein which gives a detectable signal, a sequence 5''UTR and 3''UTR sequence, wherein said sequence...
aGeneral device architecture of a perovskite solar cell.bThe distribution of stability protocols used for stability data in the Perovskite Database.cTwo possible efficiency decay curves of perovskite solar cells illustrating different types of burn-in behaviors followed by a slower exponential decay. (...
The latter can be further manifested as cell chemotaxis and niche breadth. Chemotaxis, by which cells sense chemical gradients and move directionally with preference, reflects spatiotemporal heterogeneity of energy supply; those living in stable, homogenous environments harbor fewer methyl-accepting ...
The size of the new cell is comparable to the conventional six-transistor cell of same technology and design rules. Also, the proposed cells uses a single bit-line for both read and write purposes. The cell proposed in this paper consumes less dynamic power and has higher read stability ...
Changing the position and/or type of the cation changes the channel size and properties of the zeolite, including its steric selectivity or molecular sieving effect. The positions of metal cations in the framework of a zeolite structure depend on the number of cations per unit cell; in other ...
Generating stable, high-producing mammalian cell lines is a major bottleneck in the manufacture of recombinant therapeutic proteins. Conventional gene transfer methods for cell line generation rely on random plasmid integration, resulting in unpredictable and highly variable levels of transgene expression. ...
It highlights the problems of adequate oxygen supply and a need to develop new reactors via a rational approach to process design and operation.关键词: Immobilized cell viable cell operational stability oxygen supply processing parameters DOI: 10.1016/S0141-0229(98)00046-5 年份: 1998 ...
Cell line sources MRC5 sv40 immortalized human fibroblast and mouse embryonic stem cells (mESCs) were generated in Nitika Taneja’s lab59. Stable TIG-3 human fibroblast was generated in Anja Groth’s lab31. Cell culture MRC5 human fibroblasts were cultured in a 1:1 ratio of Dulbecco’s modi...