However, mutant SHP2-transformed cell lines have not been generated. In the present study, we established oncogenic mutant SHP2-transformed cell lines from erythropoietin (EPO)-dependent HCD-57 erythroid leukemia cells. First, we generated recombinant retroviruses expressing SHP2-D61Y and SHP2-E76K...
It is practicable to use these cell lines for large-scale production of anti-P. aeruginosa MoAbs and such MoAbs must be useful for the therapeutics of patients with P. aeruginosa infection.doi:10.1111/j.1348-0421.1992.tb02132.xO'Oka, Hisayoshi...
稳定表达细胞系构建 (Stable Cell Line Establishment) 应用简介 稳定表达细胞株主要应用于以下研究中: 1)需要长期在目的细胞中研究基因功能。通过构建稳定株,可以大大降低频繁转染或者病毒包装的成本,也方便长期的实验研究; 2)表达抗体的稳定表达细胞株可以用于稳定表达所需要的抗体,为后期抗体生产提供必要条件; ...
CHO cells were transfected with the construct, and finally a stable CHO cell line expressing human recombinant Hp (rHp) was established. The expression of rHp was confirmed with RT-PCR and Western blot analyses both at mRNA and protein levels. This is the first report of establishing a stable...
of major histocompatibility complex class I on liver cells by hepatitis C virus core protein via p53 and TAP1 impairs natural killer cell cytotoxicity. Jou... Kerstin,Herzer,Christine,... - 《Journal of Virology》 被引量: 276发表: 2003年 The effect of laparotomy and laparoscopy on the estab...
The useful experimental system includes the following elements: establishment of stable cell lines, isolation and culture of single cells, and plant regeneration. Such a kind of system can be used both for screening cell mutants and for genetic manipulation. Cell lines are the excellent materials fo...
The stable cell line responded 5-6 folds more sensitively than the transiently transfected cells with Runx2. Though overexpression of any Runx gene stimulated the luciferase activity, Runx2 enhanced the reporter activity the highest. Collectively, the 6xOSE2-luc stable cells would be a good ...
Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies. 来自 NCBI 喜欢 0 阅读量: 10 摘要: Establishing specific cell lineages from human induced pluripotent stem cells (hiPSCs) is vital for cell therapy approaches in regenerative medicine, particularly for ...
establishmentofastablemdckcelllineexpressingns1protein 中国动物传染病学报 2011,l9(2):8—12 ChineseJouma1ofAnimalInfectiousDiseases ·研究论文· 稳定表达流感病毒PR8株NS1蛋白 MDCK细胞系的建立 张旭 ,滕巧泱,闫丽萍,周洁文,徐大伟,颜丕熙, 姬希文,戴晓光,王洪斌,高利,李泽君 (1.中国农业科学院上海兽医研究所...
stable expression of GFP or GFP-C-Daam1 was confirmed by Western blotting.Boyden chamber assay was used to test the intrinsic motility of the cells.Results:The cell lines stably expressing GFP or GFP-C-Daam1 were established successfully by the lentivrial infection.The GFP-C-Daam1 expressing...