稳定表达细胞系构建 (Stable Cell Line Establishment) 应用简介 稳定表达细胞株主要应用于以下研究中: 1)需要长期在目的细胞中研究基因功能。通过构建稳定株,可以大大降低频繁转染或者病毒包装的成本,也方便长期的实验研究; 2)表达抗体的稳定表达细胞株可以用于稳定表达所需要的抗体,为后期抗体生产提供必要条件; ...
We also carried out parallel experiments with the pro-B Ba/F3 cell line that require interleukin 3 (IL3) for survival. Interestingly, over-expression of SHP2-D61Y and SHP2-E76K was not sufficient to give rise to IL3-indepdent Ba/F3 cells, suggesting that HCD57 cells have some unique...
There are many different approaches for establishing stable cell lines, depending on the type of expression you’re interested in (inducible vs. constitutive) and the construct that you are incorporating. This protocol is specific for the establishment of cell lines that constitutively express GFP-...
Stable cell lines that are engineered to express a gene of interest via transgene integrations into the host genome provide an efficient approach to conduct such analysis. However, because establishment of such cell lines are tedious in procedures, time-consuming in development process, and expensive...
8.Construction of CHO Cell Line Stably Expressing Mouse Lamada-Interferon稳定表达鼠IFN-λ的CHO细胞系建立 9.Stability Analysis of Fuzzy Cellular Neural Networks Systems模糊细胞神经网络系统的稳定性分析 10.Establishment of Cell Line for Stable Expression of Ag85A Protein稳定表达Ag85A蛋白细胞系的建立 11....
The stable cell line responded 5-6 folds more sensitively than the transiently transfected cells with Runx2. Though overexpression of any Runx gene stimulated the luciferase activity, Runx2 enhanced the reporter activity the highest. Collectively, the 6xOSE2-luc stable cells would be a good ...
. Geneticin (G418 sulfate), zeocin, hygromycin B, puromycin, and blasticidin antibiotics are the most common selection antibiotics used for stable cell transfection. These antibiotics provide unique solutions for your research needs, such as dual selection and rapid, stable cell line establishmen...
Establishment, characterization, and successful adaptive therapy against human tumors of NKG cell, a new human NK cell line. Natural killer (NK) cells play important roles in adoptive cellular immunotherapy against certain human cancers. This study aims to establish a new human N... Min,Cheng,Jua...
2) Stable transfected cell line 稳定表达细胞系 例句>> 3) stable transfected CHO cell line 稳定转染的CHO细胞系 1. Construction of eukaryotic expression vector of human MCHR2 and establishment of its stable transfected CHO cell line; 目的构建人MCHR2真核表达载体,转染CHO细胞,建立稳定转染的CHO...
et al. Establishment of Stable CHO Cell Line Expressing Recombinant Human Haptoglobin: Toward New Haptoglobin-Based Therapeutics. Iran J Sci Technol Trans Sci 42, 1097–1103 (2018). https://doi.org/10.1007/s40995-017-0381-z Download citation Received27 August 2016 Accepted24 November 2017 ...