"Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in." BioTechniques 2017; 62(4). http://dx.doi.org/10.2144/000114534Chiu-An Lo, Alexander W Greben, and Brian Edwin Chen. Generating stable cell lines with quantifiable protein production using ...
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Once the Flp-In host cell line containing an integrated FRT site has been created, subsequent generation of Flp-In cell lines expressing the gene(s) of interest is rapid and efficient. The Flp-In System allows the gener...
The development of cell lines via immortalization has been proposed as a suitable strategy to eliminate batch-to-batch variability introduced by donor-derived material [28,29]. In addition, due to the adherent nature of hMSCs [30], cell yields achievable during an optimized process [31] are ...
(a) Stable cell lines were labeled with 35S methionine/cysteine for 1 h and chased for 6 h in label-free medium. FGE was immunoprecipitated from cells and medium, and quantified by densitometry after SDS-PAGE and phosphor- imaging. The results shown are representative for three independent ...
www.nature.com/scientificreports OPEN A general and efficient strategy for generating the stable enzymes received: 16 May 2016 accepted: 31 August 2016 Published: 26 September 2016 Xiao-Fei Zhang1, Guang-Yu Yang1,Yong Zhang1,Yuan Xie1, Stephen G. Withers2 &Yan Feng1 The ...
Stable lines of transgenic zebrafish exhibit reproducible patterns of transgene expression Development, 109 (1990), pp. 577-584 CrossrefView in ScopusGoogle Scholar 13 S. Isogai, N.D. Lawson, S. Torrealday, M. Horiguchi, B.M. Weinstein Angiogenic network formation in the developing vertebrate ...
When using the two-vector system, a stable cell line that expresses Cas9 must first be generated. The cell line can then be reused in multiple knockout experiments. Alternatively, a number of vendors offer Cas9-expressing cell lines, some of which have inducible Cas9 expression (https://www....
14.The processor of claim 11, wherein generating the first update and the second update comprises:determining a first set of cells included in the first update;for at least one cell included in the first set of cells, computing a transition kernel for a second set of cells within a neighbo...
(e) Plots validating the scaling law l20vi ¼ const where the constant depends on alloy composition and the dashed straight lines are linear fits; l0 increases with dealloying depth xi due to a decrease of the magnitude of the concentration gradient of B in liquid and hence vi. high ...