3. To set up for the stable cell line selection, split cells and transfect with your construct. The following day, replace the standard media with media containing G418. Over time this will select for cells that have stably incorporated the GFP plasmid into their genomic DNA. The amount ...
Product:1321N1cell line stably expressing CRISPR Cas9nuclease Description:This product is a cell line stably expressing CRISPR Cas9nuclease.The cell line also expresses the hygromycin resistance gene.In combination with separately transfected or transduced single guide RNAs(sgRNAs),this cell line will ...
1. Prepare a sterile 15-ml tube with 9 ml fresh medium without selection agents pre-warmed at 37oC.2. Thaw the TLR4 stable cell line vial quickly in a 37oC water bath, keeping the cap portion out of the water to avoid any possible contamination.3. Upon thawing, take the vial out ...
A process for the preparation of a stable cell line for the production of a specific protein, by the induction of tumours in transgenic animals and the selection of the progeny which has developed tumours. In a variant, the sequence coding for the protein is introduced into transgenic animals,...
To obtain recombinant cell lines with highly amplified genes of interest, the clones underwent dihydrofolate reductase-mediated gene amplification. Using the best strategy for the selection and amplification of mAb-producing clones, we achieved the production of more than 1 g/L in small scale, non-...
In general, the volumetric productivity of the cell pools and individual cell lines generated by transposition was stable for up to 3 months in the absence of selection. Our results indicate that the PB transposon supports the generation of cell lines with high and stable transgene expression at ...
Establishment of a WinPac-RD packaging cell line: introduction of HIV-1 Rev and an RDpro envelope The remaining vector components were introduced by a series of plasmid DNA transfection, antibiotic selection, cell cloning and clone screening (Figure 2a). Firstly, HIV-1 Rev, which is required ...
After 5 days, 800 μg ml−1 of G418 (Sigma-Aldrich) was added as a selection marker for stably transfected cells. After 4 weeks the surviving cells were FACS sorted according to their emission in the 488 nm laser line. Only cells showing clear mGFP expression were used for ...
The present investigation was carried out to study different selection parameters for foliage yield and its important yield contributing traits in 29 strai... S Shukla,A Bhargava,A Chatterjee,... - 《Plant Foods for Human Nutrition》 被引量: 149发表: 2006年 Diversity in phenotypic and nutrition...
Single-cell RNA-seq (scRNASeq) has become a powerful technique for measuring the transcriptome of individual cells. Unlike the bulk measurements that average the gene expressions over the individual cells, gene measurements at individual cells can be use