Generation of stable cell lines by spontaneous immortalization of primary cultures of porcine granulosa cells. Mol Reprod Dev 2000;57:366-374.Gillio-Meina C, Swan CL, Crellin NK, Stocco DM, Chedrese PJ: Generation of stable cell lines by spontaneous immortalization of primary cultures of porcine...
Insect cell culture is becoming increasingly important for applications including recombinant protein production and cell-based screening with chemical or RNAi libraries. While stable mammalian cell lines expressing a protein of interest can be efficient
Generating stable, high-producing mammalian cell lines is a major bottleneck in the manufacture of recombinant therapeutic proteins. Conventional gene transfer methods for cell line generation rely on random plasmid integration, resulting in unpredictable and highly variable levels of transgene expression. ...
Advantages of the Flp-In™ T-REx™ System Use of the Flp-In™ T-REx™ System to generate stable expression cell lines provides a number of advantages as described below: Once the Flp-In™ T-REx™ ...
Our data show high yields and product quality homogeneity of stable transfected pools and cell lines that significantly improve upon, or at the very least match highest reported expression and/or functional activity levels for both these proteins. We, therefore, conclude that STEP® technology is ...
Transient transformation of tobacco leaf epidermal cells is a relatively fast technique to assess expression of genes of interest. These cells can be used to generate stable plant lines using a more time-consuming, cell culture technique. Transient expression takes from 2 to 4 days whereas stable ...
Cell-based therapeutics are an emerging modality with the potential to treat many currently intractable diseases through uniquely powerful modes of action. Despite notable recent clinical and commercial successes, cell-based therapies continue to face numerous challenges that limit their widespread translation...
Immortalized mouse odontoblast cell line MO6-G3 application for in vitro biocompatibility testing. PURPOSE: This study was designed to determine the usefulness of an established stable immortalized mouse odontoblast cell line (MO6-G3) for dental material... M Macdougall,JK Selden,JR Nydegger,....
The therapeutic efficacy of whole tumor cell vaccines (TCVs) is modest, which has delayed their translation into personalized immunotherapies in the clinic. Here, we develop a TCV platform based on photothermal nanoparticle-loaded tumor cells, which can be rationally applied to diverse tumor types ...
Fig. 1: Timeline for the number of components on a silicon photonic integrated circuit (PIC) over generations of small-scale, medium-scale, large-scale, and very-large-scale integration (SSI, MSI, LSI, VLSI, respectively). A component is a unit cell that is combined with other unit cells...