另外,ICLAC(The International Cell Line Authentication Committee)提倡对新建立的原代细胞系或未被收录STR数据的细胞系进行STR分型、共享STR数据, 一来可以为同行们提供数据参考,二来可以有效控制细胞系的交叉污染和错误辨识。误区二:细胞系进入实验室时做过STR鉴定,此后就不必再做细胞系不管是来自于权威细胞库还...
格式如下:The cell lines were characterized byGenetic Testing Biotechnology Corporation (Suzhou,China)using short tandem repeat (STR) markers. Selected high quality publications using our cell line authentication
苏州鉴达(Genetic Testing Biotechnology, GTB)采用STR分型技术,针对人源细胞23个基因座、小鼠源细胞19个基因座(18个小鼠源基因座+1个人源基因座TH01)进行检测,3130XL型遗传分析仪进行毛细管电泳,与DSMZ、Cellosaurus、ATCC等数据库进行比对,出具权威英文报告,为全国近500家科研机构(高校、研究所、医院、公司)提供高...
a Genetic diagram of the iSLC strain. b Simulations of the mathematical model showing three different dynamics at low (blue), medium (orange) and high (purple) inducer values. Medium values are predicted to result in sustained oscillations. Left: steady state maximum and minimum cell population ...
Investigating the role that host erythrocyte proteins play in malaria infection is hampered by the genetic intractability of this anucleate cell. Here we report that reticulocytes derived through in vitro differentiation of an enucleation-competent immortalized erythroblast cell line (BEL-A) support both...
5.43.2.2.2Cell line Cell lineis a general term that applies to a defined population of cells that can be maintained in culture for an extended period of time, retaining stability of certain phenotypes and functions. Cell lines are usually clonal, meaning that the entire population originated fro...
The cell lines may mutate, and genetic drift or (usually unknown) selective pressures in culture may promote significant changes in phenotype; for example, PC12 cells from different laboratories may show different reponses compared to the original line (Dixon et al. 2005; Yoshio et al. 2001)....
苏州鉴达(Genetic Testing Biotechnology, GTB)自行研发小鼠细胞STR分型试剂盒,可提供小鼠细胞鉴定服务。试剂盒可对小鼠细胞18个基因座(4-2、6-4、1-1、6-7、2-1、17-2、11-2、8-1、19-2、7-1、1-2、13-1、5-5、12-1、18-3、15-3、3-2、X-1)进行多重PCR。此外,试剂盒包含一个人源STR基因...
The genetic aberrations of cell lines related with increasing passage numbers, The genotypic and phenotypic drift in continuous cultures, especially deposited in cell banks for many years, The cell line response toward the tested drug might be different form patient response toward the same drugs, Di...
Genetic Characteristics of the Human Hepatic Stellate Cell Line LX-2 ¨ rg Weimer2., Steffen K. Meurer1, Anja Kron3, Barbara Seipel3, Inga Vater4, Ralf Weiskirchen1*., Jo 2 Norbert Arnold , Reiner Siebert4, Lieming Xu5, Scott L. Friedman6, Carsten Bergmann3,7,8 1 Institute of Clin...