et al. Match criteria for human cell line authentication: where do we draw the line? Int. J. Cancer 132, 2510–2519 (2013). Article CAS PubMed Google Scholar Baker, D. et al. Detecting genetic mosaicism in cultures of human pluripotent stem cells. Stem Cell Rep. 7, 998–1012 (2016...
” Furthermore, inherent cell line characteristics such as the gender, ethnicity and detectedgenetic mutationsmay affect the suitability of using certain lines for addressing specific questions. Depending on the differentiation protocol, certain cell lines may show a preference for distinct differentiation ...
Cell lines should be obtained from a reliablegenetictoxicology laboratory that has maintained and characterized them appropriately as described here. Some cultivars (sublines) may be inappropriate for genetictoxicology testingbecause of genetic instability; the WBL clone ofCHO cellshas given good results ...
as well as ofHadh.e, Smoothed gene expression trends along the delta lineage for all 12,987 genes that are expressed in at least ten cells, clustered using louvain68. Cluster 1 contains transiently upregulated genes. The solid line denotes mean...
2.1. Genetic Circuits Cells develop a set of regulatory tools to sense and process stimuli (information) from the external environment and internal physiological states [23]. In response to constant environmental changes, cellular activities are tuned through a set of regulatory elements, controlling ...
Creative Bioarray offers a wide range of cell line testing and assays from cell viability and proliferation to cellular phosphorylation assays.
The genetic aberrations of cell lines related with increasing passage numbers, The genotypic and phenotypic drift in continuous cultures, especially deposited in cell banks for many years, The cell line response toward the tested drug might be different form patient response toward the same drugs, Di...
Various protocols have been developed all of which follow the same basic steps: iPSC generation by incorporating pluripotent genetic factors, self-renewal and expansion of the iPSCs, and differentiation into erythroid lineage cells, which includes commitment, expansion, and maturation [27, 31, 48,49...
Primary fibroblasts are a type of finite cell line widely used as model systems because they are easily isolated and grown in culture, and almost never show genetic alterations in oncogenes or tumor suppressors [16, 17]. LCLs are among the most widely created, archived, and analyzed continuous...
the relationships between the different RPC sub-populations, and their relevance to RPC competence for individual retinal fates, have only begun to be addressed. Conventional experimental approaches have provided much insight into the genetic pathways and mechanisms underlying the formation of individual re...